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Monoclonal Anti-Insulin antibody produced in mouse

clone K36AC10, ascites fluid

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Synonym(s):
Insulin Antibody, Insulin Antibody - Monoclonal Anti-Insulin antibody produced in mouse
MDL number:
MFCD00164675
NACRES:
NA.41

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

K36AC10, monoclonal

contains

15 mM sodium azide

species reactivity

feline, human, rat, horse, rabbit, guinea pig, bovine, monkey, sheep, canine, pig

technique(s)

dot blot: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000 using human or animal pancreas
radioimmunoassay: suitable

isotype

IgG1

UniProt accession no.

P01308

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... INS(3630)

General description

The antibody reacts specifically against insulin by RIA and immunocytochemistry. It exhibits cross-reactivity with human proinsulin. The antibody binds to insulin with an affinity constant of 8.8 × 109 M-1 in RIA.
The insulin gene is mapped to human chromosome 11p15.5.

Application

Monoclonal Anti-Insulin antibody produced in mouse has been used in immunoblotting assay and ELISA.
Monoclonal Anti-Insulin antibody produced in mouse was used in ELISA-based protein pin array assay to study the interactions between human αB crystalline and insulin. It was used for flow cytometric analysis of rat white blood cells and mast cells.

Biochem/physiol Actions

Insulin is a major regulatory hormone important in the metabolic processes, transport of glucose, amino acids and certain ions. It is also involved in the synthesis of nucleic acids and proteins. The biological effects of insulin are mediated by insulin receptor and it integrates multiple major signaling pathways.
Insulin is a major regulatory hormone important in the metabolic processes, transport of glucose, amino acids and certain ions. It is also involved in the synthesis of nucleic acids and proteins. The biological effects of insulin are mediated by insulin receptor and it integrates multiple major signaling pathways. Abnormal insulin secretion or function leads to diabetes mellitus, obesity and other carbohydrate metabolism disorders. Insulin is known to stimulate cell division.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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H Keilacker et al.
Biomedica biochimica acta, 45(8), 1093-1102 (1986-01-01)
We investigated four insulin-specific hybridoma antibodies with respect to their kinetic properties as well as the binding behaviour of some combinations of them. From equilibrium binding data all but one antibodies were shown to bear homogeneous binding sites. They revealed
Sandra K Szlapinski et al.
The Journal of physiology, 597(16), 4237-4250 (2019-06-18)
Pancreatic β-cell dysfunction is hypothesized to be the significant determinant of gestational diabetes pathogenesis, however pancreatic samples from patients are scarce. This study reports a novel mouse model of gestational glucose intolerance in pregnancy, originating from previous nutrition restriction in
Proinsulin-specific monoclonal antibodies. Immunocytochemical application as beta-cell markers and as probes for conversion.
O D Madsen
Diabetes, 36(10), 1203-1211 (1987-10-01)
Rebecca L Hull et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 60(10), 749-760 (2012-07-24)
The pancreatic islet comprises endocrine, vascular, and neuronal cells. Signaling among these cell types is critical for islet function. The extracellular matrix (ECM) is a key regulator of cell-cell signals, and while some islet ECM components have been identified, much
J Matthew Rhett et al.
Journal of diabetes research, 2016, 7262680-7262680 (2016-01-21)
Total pancreatectomy and islet autotransplantation is a cutting-edge technique to treat chronic pancreatitis and postoperative diabetes. A major obstacle has been low islet cell survival due largely to the innate inflammatory response. Connexin43 (Cx43) channels play a key role in
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