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I2411

Isocitrate Dehydrogenase 1 (NADP+) human

recombinant, expressed in Sf9 cells, ≥90% (SDS-PAGE)

Synonym(s):

IDH1, Isocitrate Dehydrogenase Cytoplasmic

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About This Item

CAS Number:
9028-48-2
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
1.1.1.42 (BRENDA, IUBMB)
Assay:
≥90% (SDS-PAGE)
Recombinant:
expressed in Sf9 cells
Concentration:
≥0.30 mg/mL protein (Bradford)
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recombinant

expressed in Sf9 cells

Quality Segment

assay

≥90% (SDS-PAGE)

form

buffered aqueous glycerol solution

mol wt

47.6 kDa

concentration

≥0.30 mg/mL protein (Bradford)

NCBI accession no.

relevant disease(s)

cancer (lung)

shipped in

dry ice

storage temp.

−70°C

General description

Human Isocitrate Dehydrogenase (IDH1), also known as oxalosuccinate decarboxylase, full length [amino acids 1-414 (end)] with C-terminal FLAG-tag, expressed in Sf9 cells via a baculovirus expression system.

Application

Human Isocitrate Dehydrogenase (IDH1) is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. It may be useful as a biomarker for diagnosis and prognosis prediction of non-small cell lung cancer .

Biochem/physiol Actions

Human Isocitrate Dehydrogenase (IDH1), also known as oxalosuccinate decarboxylase, is a full length amino acid with a C-terminal FLAG-tag and is expressed in Sf9 cells via a baculovirus expression system. IDH1 expression has been correlated with poor survival of non-small cell lung cancer. IDH1 promotes tumor growth .

Physical form

Solution in 45 mM Tris-HCl, 124 mM NaCl, 2.4 mM KCl, 90 μg/ml FLAG peptide, 3 mM DTT, and 10% glycerol at pH 8.0.

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Storage Class

10 - Combustible liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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