I3909
Ionomycin calcium salt
Ready Made Solution, from Streptomyces conglobatus, 1 mM in DMSO
biological source
Streptomyces conglobatus
Quality Level
Assay
≥98% (HPLC)
shelf life
2 yr
storage condition
(Tightly closed. )
concentration
1 mM in DMSO
color
colorless
mp
18.4 °C
solubility
DMSO: 1 mM
antibiotic activity spectrum
Gram-positive bacteria
Mode of action
cell membrane | interferes
shipped in
wet ice
storage temp.
−20°C
SMILES string
C[C@@H]1CCC(=O)O[Ca]OC(=CC(=O)[C@@H](C)C[C@@H](C)C1)[C@H](C)C[C@H](C)C\C=C\[C@@H](C)[C@@H](O)[C@@H](C)[C@@H](O)C[C@@H]2CC[C@](C)(O2)[C@H]3CC[C@](C)(O3)[C@@H](C)O
InChI
1S/C41H72O9.Ca/c1-25(21-29(5)34(43)24-35(44)30(6)22-27(3)20-26(2)14-15-38(46)47)12-11-13-28(4)39(48)31(7)36(45)23-33-16-18-41(10,49-33)37-17-19-40(9,50-37)32(8)42;/h11,13,24-33,36-37,39,42-43,45,48H,12,14-23H2,1-10H3,(H,46,47);/q;+2/p-2/b13-11+,34-24-;/t25-,26-,27+,28-,29-,30+,31+,32-,33+,36+,37-,39-,40+,41+;/m1./s1
InChI key
WKRWUYKLUMMAKG-WYGBAUISSA-L
Related Categories
General description
Ionomycin is commonly used in cell biology experiments to induce a rapid and controlled increase in intracellular calcium levels. This allows to study the downstream effects of calcium signaling pathways, such as the activation of enzymes, the release of hormones, and the changes in gene expression.Ionomycin is also used to investigate the role of calcium in specific cellular processes, such as cell death, immune cell activation, and muscle contraction. For example, ionomycin can be used to induce apoptosis in cancer cells, study the activation of T cells, and investigate the role of calcium in muscle contraction.
Application
- Study of calcium signaling and its role in various biological processes
- Investigation of intracellular calcium levels, calcium-dependent enzymes, and cellular responses triggered by calcium influx
- Induction of a rapid and controlled increase in intracellular calcium levels in cell biology experiments
- Elucidation of specific cellular mechanisms and signaling pathways in combination with other substances, such as fluorescent calcium indicators or protein kinase activators
- to induce central demyelination
- inhibit adrenal bovine TREK-1 channels
- to regulate cell division of mature human B cells
Biochem/physiol Actions
Mode of Action: Facilitates the transfer of calcium ions (Ca2+) across biological membranes
Antimicrobial Spectrum: Active against Gram-Positive bacteria
Features and Benefits
- Ready available solution reduce the need for preparation time
- Efficient choice for studying calcium signaling and its role in various biological processes
- Allows for rapid and controlled manipulation of intracellular calcium levels
- Versatile and widely used in cell biology research
Other Notes
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
188.6 °F - closed cup
Flash Point(C)
87 °C - closed cup
Personal Protective Equipment
Certificates of Analysis (COA)
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How does the storage temperature relate to shipping conditions?
The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment. See Shipping and Storage for more information.
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Is Product No. I3909 (Ionomycin calcium salt solution) hygroscopic?
Yes, this product is hygroscopic. It is packaged under an inert gas. It is recommended to store I3909 under an inert gas and at -20°C protected from light.
What is the concentration of the Ionomycin calcium salt solution, Product No. I3909?
It is a 1mM solution or ~ 0.75mg/mL in DMSO.
Has Ionomycin calcium salt solution, Product No. I3909, been tested for use in Cell Culture applications?
Sigma Aldrich has not tested this material for use in cell culture. However, several references for use of ionomycin in cell culture applications can be found in the product information sheet (under Documents, above).
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Articles
This study presents an example of a typical ELISpot assay for quantifying the number of pre-existing, antigen-reactive T cells from peripheral blood mononuclear cell (PBMC) samples obtained from donors previously infected with cytomegalovirus (CMV).
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