I6277
Anti-Interleukin-2 Soluble Receptor β antibody produced in goat
affinity isolated antibody, lyophilized powder
Synonym(s):
Anti-IL-2 sRβ
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About This Item
UNSPSC Code:
51111800
MDL number:
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ELISA (i), Neutral, WB
Citations:
5
biological source
goat
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
lyophilized powder
species reactivity
human
technique(s)
indirect ELISA: suitable, neutralization: suitable, western blot: suitable
UniProt accession no.
storage temp.
−20°C
Gene Information
human ... IL2RB(3560)
General description
Interleukin-2 (IL-2) is a cytokine produced by activated T cells in response to antigen stimulation. IL-2 is important for proliferation of lymphocytes, natural killer cells and macrophages and in the differentiation of CD4+ cells into Th1 and Th2 effector cells. The effects of IL-2 are mediated by the signalling activated by its binding to IL-2 receptor (IL-2 R). IL-2 R which contains three membrane subunits: α, β and γ. The β and the γ subunits complex results in the binding of IL-2 with higher affinity. IL-2 R β is expressed on resting monocytes and B cells, CD8+ and CD4+T cells, NK cells and neutrophils and mature thymocytes. IL-2 R activates key signalling pathways such as MAPK, JAK/STAT as well as PI3K/Akt. Activation of these pathways modulates production of inflammatory cytokines, survival and progression through cell cycle
Anti-Human IL-2 soluble receptor β (IL-2 sRβ) specifically reacts with human IL-2 Rβ. The antibody shows less than 15% cross reactivity with recombinant human IL-3 sRα, less than 5% cross reactivity with recombinant human IL-1 RII, IL-2 sRγ, IL-5 sRα, and less than 2% cross reactivity with recombinant human IL-2 sRα, IL-4 sR, and IL-5sRβ. In addition, the antibody shows no cross reactivity with other cytokines tested.
Anti-Human IL-2 soluble receptor β (IL-2 sRβ) specifically reacts with human IL-2 Rβ. The antibody shows less than 15% cross reactivity with recombinant human IL-3 sRα, less than 5% cross reactivity with recombinant human IL-1 RII, IL-2 sRγ, IL-5 sRα, and less than 2% cross reactivity with recombinant human IL-2 sRα, IL-4 sR, and IL-5sRβ. In addition, the antibody shows no cross reactivity with other cytokines tested.
Immunogen
recombinant human IL-2 soluble receptor β (IL-2 sRβ) expressed in Sf 21 insect cells.
Application
Anti-Human IL-2 soluble receptor β (IL-2 sRβ) antibody may be used for immunoblotting at a working concentration of 1-2 μg/ml. For indirect ELISA, the recommended concentration is 0.5-1.0 μg/ml. The antibody is suitable for IL-2 sRβ neutralization reactions at a working antibody concentration of 10-30 μg/ml.
Biochem/physiol Actions
The antibody will neutralize human cell surface IL-2 receptor β mediated-bioactivity. For cells expressing high affinity IL-2 receptors, use of both anti-IL-2 Rα and anti-IL-2 Rβ is recommended. The antibody shows ≤15% cross-reactivity with recombinant human IL-3 sRα, ≤5% cross-reactivity with recombinant human IL-1 RII, IL-2 sRγ, IL-5 sRα, and ≤2% cross-reactivity with recombinant human IL-2 sRα, IL-4 sR, and IL-5 sRβ.
Physical form
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline.
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Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Matthew A Williams et al.
Nature, 441(7095), 890-893 (2006-06-17)
Although interleukin-2 (IL-2) was initially characterized as the primary T-cell growth factor following in vitro activation, less is known about its role in shaping T-cell responses to acute infections in vivo. The use of IL-2- or IL-2-receptor-deficient mice is problematic
Hans Dooms et al.
Journal of immunology (Baltimore, Md. : 1950), 172(10), 5973-5979 (2004-05-07)
The acquisition of long-term survival potential by activated T lymphocytes is essential to ensure the successful development of a memory population in the competitive environment of the lymphoid system. The factors that grant competitiveness for survival to primed T cells
B H Nelson et al.
Advances in immunology, 70, 1-81 (1998-10-02)
Studies of the biology of the IL-2 receptor have played a major part in establishing several of the fundamental principles that govern our current understanding of immunology. Chief among these is the contribution made by lymphokines to regulation of the
Arian Laurence et al.
Immunity, 26(3), 371-381 (2007-03-17)
Recent work has identified a new subset of effector T cells that produces interleukin (IL)-17 known as T helper 17 (Th17) cells, which is involved in the pathophysiology of inflammatory diseases and is thought to be developmentally related to regulatory
Katrina K Hoyer et al.
Immunological reviews, 226, 19-28 (2009-01-24)
Interleukin-2 (IL-2) has multiple, sometimes opposing, functions during an inflammatory response. It is a potent inducer of T-cell proliferation and T-helper 1 (Th1) and Th2 effector T-cell differentiation and provides T cells with a long-lasting competitive advantage resulting in the
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