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Merck
CN

I6529

IMDM

With sodium bicarbonate and ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

Iscove′s MDM, Iscoves MDM, Iscove’s Modified Dulbecco’s Medium, IMDM

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75
MDL number:
Technical Service
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Product Name

Iscove′s Modified Dulbecco′s Medium, With sodium bicarbonate and L-glutamine, liquid, sterile-filtered, suitable for cell culture, suitable for hybridoma

Quality Segment

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

impurities

endotoxin, tested

components

glucose: yes
phenol red: yes
NaHCO3: yes
L-glutamine: yes

shipped in

ambient

storage temp.

2-8°C

General description

Iscove′s Modified Dulbecco′s Medium contains selenium, additional amino acids and vitamins, sodium pyruvate, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer, and potassium nitrate instead of ferric nitrate, and is a modification of Dulbecco′s Modified Eagle′s medium (DME). Iscove′s medium can be used to support murine B lymphocytes, bone marrow hemopoietic tissue, B cells stimulated with lipopolysaccharide, T lymphocytes, and various hybrid cells.

Application

Iscove′s Modified Dulbecco′s Medium has been used to culture:
  • human hepatocellular carcinoma (HCC) cell line HepG2 as monolayers
  • to culture synovial tissue samples
  • bacterial strain inoculum for stimulation of immune cell experiments



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Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable



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