Monoclonal Anti-Interleukin-6 antibody produced in mouse

Interleukin-6 is a pleotropic cytokine involved in hematopoiesis, inflammation and immune response. IL-6 is secreted by macrophages, dendritic cells and B cells, in response to TNF-α, PGDF and IL-1. The signaling by IL-6 is mediated by heterodimeric receptor, IL-6R, comprising of IL-6Rα (soluble factor) and gp130 (sometimes called IL-6Rβ). gp130 is crucial component for downstream signal transduction. In response to IL-6, gp130 activates kinases of JAK family, STATs, MAPK and Ras proteins. IL-6-mediated signaling is essential for normal development of T and B cells and has been implicated in many diseases such as rheumatoid arthritis, Crohn disease, sepsis, fever, systemic lupus erythematosus, osteoporosis and insulin resistance. Monoclonal Anti-Interleukin-6 antibody recognizes human IL-6. The antibody shows no cross-reactivity with recombinant mouse IL-6 or recombinant human IL-6 sR, OSM, LIF CNTF, or IL-11 or recombinant horse gp130.

recombinant human IL-6.

Monoclonal Anti-Interleukin-6 antibody may be used for ELISA at a minimum antibody concentration of 4 μg/ml. For immunoblotting a working antibody concentration of 1-2 μg/ml may be used. The antibody may be used in neutralization assays; ND50 is 0.01-0.15 μg/ml. For neutralization of IL-6 from human peripheral blood mononuclear cells, a concentration of 2.5 μg/ml was used.

The antibody will neutralize the biological activity of recombinant human IL-6. The antibody shows no cross-reactivity with recombinant mouse IL-6 or recombinant human IL-6 sR, OSM, LIF CNTF, or IL-11 or recombinant horse gp130.

Lyophilized from 0.2 μm filtered solution in phosphate buffered saline.

Purified using protein A.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.