Skip to Content
Merck
CN

Key Documents

View All Documentation

I8523

Interleukin-12 from mouse

≥97% (SDS-PAGE), recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture

Synonym(s):

IL-12

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View

About This Item

UNSPSC Code:
12352202
NACRES:
NA.32
MDL number:
MFCD00212975
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

biological source

mouse

Quality Segment

100

recombinant

expressed in baculovirus infected Sf21 cells

assay

≥97% (SDS-PAGE)

form

lyophilized powder

potency

0.01-.1 ng/mL ED50

mol wt

glycoprotein 70 kDa

packaging

pkg of 10 μg

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

UniProt accession no.

P43431, P43432

storage temp.

−20°C

Gene Information

mouse ... Il12a(16159), Il12b(16160)

Application

Interleukin-12 (IL-12) from mouse has been used to study the effect of IL-12 on Th1 (type 1 T helper) immunity in Mongolian gerbils (Meriones unguiculatus) infected with Sporothrix schenckii. It has been used as a standard in ELISA (enzyme-linked immunosorbent assay) cytokine assay.

Biochem/physiol Actions

Interleukin-12 (IL-12) was identified as a factor secreted by human Epstein-Barr (EBV)-transformed B cell lines. IL-12, or Natural Killer Cell Stimulatory Factor (NKSF), is a 75 kDa disulfide-linked heterodimer of a 35 kDa subunit and 40 kDa subunit. IL-12 is produced predominantly by monocytes and NK cells and induces T cells and NK cells to produce IFN-γ. Human IL-12 is not active on mouse cells, but murine IL-12 is active on both murine and human lymphocytes. The biological activity of recombinant human IL-12 was measured by its ability to stimulate the proliferation of PHA-activated human T lymphoblasts. IL-12 has anti-angiogenic properties.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.5 mg bovine serum albumin.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library