KEM0033
T4 DNA Polymerase
Ultra-pure enzyme for nucleic acid modifications
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grade
Molecular Biology
assay
>99% (SDS-PAGE)
form
buffered aqueous solution
specific activity
5,555 U/mg
concentration
3,000 U/mL
shipped in
dry ice
storage temp.
−20°C
General description
T4 DNA Polymerase catalyzes the extension of a primed DNA template in the 5′ → 3′ direction. This enzyme exhibits a powerful 3′ → 5′ exonuclease activity, while lacking any inherent 5′ → 3′ exonuclease or strand displacement functions.
Application
Suitable for DNA end-repair by fill-in of 5′ overhang and removal of 3′ overhang.
Features and Benefits
- Ultra-purification process for ultimate enzyme performance
- Highest quality specifications for ultimate product consistency
- Undetectable DNA and nuclease contamination
Physical form
Supplied in 100 mM KPO4, 1.0 mM DTT, 0.1 mM EDTA, and 50% glycerol at pH 6.5 @ 25° C.
Other Notes
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-precipitable material in 30 minutes at 37° C.
Source of protein: Purified from a strain of E. coli that expresses the recombinant T4 DNA Polymerase gene.
Supplied with:KEM0042B (10X Blue Buffer)
Unit size: 2,000 U
Storage Class
10 - Combustible liquids
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