KMM-201NV
KOD One™ PCR Master Mix -BLUE-
Ready-to-use 2X hot-start PCR master mix with a modified KOD DNA polymerase optimized for ultra-fast and convenient high-fidelity PCR
biological source
Escherichia coli (carrying the cloned UKOD DNA polymerase gene)
Quality Level
form
liquid
usage
sufficient for 25 reactions
sufficient for 500 reactions
feature
Fast PCR
High Fidelity PCR
dNTPs included
hotstart
technique(s)
PCR: suitable
input
crude DNA
shipped in
dry ice
storage temp.
−20°C
General description
This hot start KOD polymerase is antibody-inactivated with two types of antibodies that prevent the 5′→3′ polymerase activity and 3′→5′ exonuclease activity. Upon heat-activation, the 3′→5′ proof-reading ability of the enzyme generates blunt-end PCR products.
Application
- Genomic DNA amplification
- cDNA amplification
- Direct PCR
- Colony PCR
- Amplification of NGS libraries
- Site direct gene mutation
Compatible Sample Types: Serum, Plasma, Cell, Plant, soil extraction, DNA, nail, hair etc.
Sample Volume Needed: about 50ng DNA
Features and Benefits
- Fast: Amplifies the targets using the following very short conditions:
o 1∼ 10 kb: 5 sec/ kb
o 10 kb∼: 10 sec/ kb
- Ready-to-use: 2X premixed solution of UKOD DNA polymerase, buffer, dNTPs, and MgCl2. KOD One PCR Master Mix Blue includes a loading dye (BPB) to allow direct loading onto agarose gels.
- High Fidelity: Approximately 80-fold higher fidelity than conventional Taq DNA polymerase. This mix can be used for various purposes where this would be an advantage (e.g., in the preparation of long target amplicons for sequencing).
- High Efficiency: Enables high throughput PCR and ultra-fast PCR cycling conditions with an extension time of as quick as 5 sec/kb.
- Crude Sample PCR: Effective for direct PCR amplification of crude samples such as biological samples, food samples, soil extract, etc.
- High flexibility: Amplifies templates containing uracils (dU). Also, amplifies fragments using degenerate primers with inosines (dI) and uracils (dU)
Packaging
KMM-201NVS - 0.25ML is sufficient for 25 reactions at 20 μL each or for 10 reactions at 50 μL each.
Storage and Stability
Other Notes
Legal Information
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Met. Corr. 1
Storage Class Code
8B - Non-combustible, corrosive hazardous materials
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Protocols
Evaluate PCR inhibitors and enzyme selection using GenElute™-E Single Spin Plant Extraction Kit, KOD-One™ PCR Master Mix, and competitors.
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