L020000
TCA Deblock
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About This Item
NACRES:
NA.21
UNSPSC Code:
41116105
type
for DNA synthesis, for DNA synthesis
Quality Segment
storage condition
dry at room temperature
concentration
28-32 g/L (TCA content, titration)
impurities
≤200 ppm water content (Karl Fischer)
color
clear
Preparation Note
Trichloroacetic acid / Dichloromethane 3/100 (v/v)
Other Notes
Deblock solution containing trichloroacetic acid (TCA) in dichloromethane removes the dimethoxytrityl (DMT) protecting group from the 5′hydroxyl moiety of nucleotides already incorporated into the growing nucleic acid, prior to the addition of the next phosphoramidite. Removal of the DMT allows the unprotected 5′hydroxyl moiety to react with a new phosphoramidite in a subsequent extension reaction.
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Signal Word
Danger
Hazard Codes
Precautionary Statements
Hazard Classifications
Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Skin Irrit. 2 - STOT SE 3
Target Organs
Central nervous system, Respiratory system
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK
WGK 2
Flash Point (°F)
212.0 °F - closed cup
Flash Point (°C)
100 °C - closed cup
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