L0302511
ETT Activator
Synonym(s):
5-(Ethylthio)-1H-tetrazole, 5-(Ethylthio)tetrazole solution
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About This Item
type
for DNA synthesis
Quality Level
product line
Proligo Reagents
packaging
pkg of 4 × 2.5 L L0302511-04
concentration
39-43 g/L (titration)
impurities
≤30 ppm water content (Karl Fischer)
color
clear
SMILES string
CCSc1nnn[nH]1
InChI
1S/C3H6N4S/c1-2-8-3-4-6-7-5-3/h2H2,1H3,(H,4,5,6,7)
InChI key
GONFBOIJNUKKST-UHFFFAOYSA-N
General description
ETT activator is an activator solution containing 5-(Ethylthio)-1H-tetrazole – a powerful activator – in acetonitrile. It is mixed with phosphoramidite during the extension step in which it reacts with the amidite group to form a highly reactive intermediate. The intermediate then forms an internucleotide bond with the detritylated 5′hydroxyl group of the growing oligonucleotide chain.
Application
ETT activator has been used in the fast and low-cost synthesis of photolithographic DNA microarrays.
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Danger
Hazard Classifications
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 2 - Skin Irrit. 2
Storage Class
3 - Flammable liquids
wgk
WGK 3
flash_point_f
35.6 °F
flash_point_c
2 °C
Regulatory Information
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Matej Sack et al.
Journal of nanobiotechnology, 14, 14-14 (2016-03-05)
DNA microarrays are a core element of modern genomics research and medical diagnostics, allowing the simple and simultaneous determination of the relative abundances of hundreds of thousands to millions of genomic DNA or RNA sequences in a sample. Photolithographic in
Global Trade Item Number
| SKU | GTIN |
|---|---|
| L030100-4X2.5L | 04061838071507 |