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L1754

Sigma-Aldrich

Lipase from Candida rugosa

Type VII, ≥700 unit/mg solid

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Synonym(s):
Triacylglycerol acylhydrolase, Triacylglycerol lipase
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
NACRES:
NA.54

biological source

fungus (candida rugosa)

Quality Level

type

Type VII

form

lyophilized

specific activity

≥700 unit/mg solid

storage condition

(Tightly closed. Dry)

technique(s)

cell based assay: suitable

color

beige
white

solubility

water: slightly soluble

storage temp.

2-8°C

InChI

1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;

InChI key

QWZUIMCIEOCSJF-CHHCPSLASA-N

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General description

Research area: Cell Structure

Lipases are found in pancreatic secretions. This class of enzymes contains α and β hydrolase folds. Candida rugosa produces multiple lipase isoenzymes that have 80% sequence homology.

Application

Lipase from Candida rugosa has been used:
  • to synthesize dextran fatty acid esters
  • to study the effect of lipase on conjugated linoleic acid (CLA1 and CLA2) production in the presence of sunflower oil and castor oil
  • to prepare pH-imprinted enzyme for lipase-catalyzed transesterification of dextran T-40 with vinyl decanoate

Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.

Biochem/physiol Actions

Lipases are enzymes and biocatalysts that catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids. They are useful for biotransformation reactions. It possesses broad substrate tolerance and is highly stereo- and regioselective. Lipases mediate lipid transport. Elevated levels of serum lipase are observed in pancreatitis. Lipases are the preferred catalysts for long-chain fatty acids. Lipases can be used in a wide range of solvents, in both aqueous and non-aqueous media, and thus have found use in applications like organic synthesis.
Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate).

Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.

Unit Definition

One unit will hydrolyze 1.0 microequivalent of fatty acid from a triglyceride in 1 hr at pH 7.2 at 37 °C. (This is equivalent to approx. 10 microliters of CO2 in 30 minutes.)

enzyme

Product No.
Description
Pricing

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

常规特殊物品

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  4. What is the stability of Product L1754, Lipase from Candida rugosa solutions, and how should they be stored?

    Solutions in 50% glycerol may be stored at -20°C up to six months. Avoid repeated freeze/thaw cycles. It is best to store aliquots in working volumes.

  5. What is the solubility of Product L1754, Lipase from Candida rugosa?

    This product is soluble in water at 1 mg/mL.

  6. What is the difference between Candida rugosa and Candida cylindracea?

    These organisms are identical strains. C. rugosa was assigned to what was previously named C. cylindracea by the ATCC.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Ashok Kumar et al.
Biological procedures online, 18, 2-2 (2016-01-15)
Lipases are industrial biocatalysts, which are involved in several novel reactions, occurring in aqueous medium as well as non-aqueous medium. Furthermore, they are well-known for their remarkable ability to carry out a wide variety of chemo-, regio- and enantio-selective transformations.
Conjugated linoleic acid conversion by six Lactobacillus plantarum strains cultured in MRS broth supplemented with sunflower oil and soymilk.
Li, et al.
Journal of Food Science, 77, M330-M336 (2017)
Takeshi Furuhashi et al.
Frontiers in molecular biosciences, 7, 116-116 (2020-07-23)
Cellular volatile organic compounds (VOCs) are unique compounds whose metabolic pathways remain enigmatic. To elucidate their metabolism, we investigated the VOCs of lung cancer A549 and 2 non-cancer lung cells (HLB; HBEpC). Neutral sugars and lactate in the medium were
Lipase
Pirahanchi Y and SharmaS
Biochemistry (2019)
Enzymatic synthesis of oligo- and polysaccharide fatty acid esters.
van den Broek, et al.
Carbohydrate Polymers, 93, 65-72 (2013)

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