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Merck
CN

L2025

Sigma-Aldrich

Luria Agar Base, Miller

Tryptone, yeast extract, sodium chloride and agar

Synonym(s):

LA, modified, Luria-Bertani media, modified, Lysogeny broth, modified

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About This Item

UNSPSC Code:
41106200
eCl@ss:
42040102
NACRES:
NA.72
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Quality Level

form

powder

technique(s)

microbiological culture: suitable

application(s)

agriculture
microbiology

General description

Luria Bertani (LB) medium is one of the complex media that is widely used to grow Escherichia coli in small-scale cultures.

Application

Luria Agar Base, Miller has been used as a growth medium during plasmid amplification. It has also been used as a culture medium for strains of Penicillium sp. laika.
Luria broth/agar (LA), Miller (L1900) is a bacteria growth mixture of sodium chloride (0.5g/L); tryptone (10g/L) yeast extract (5g/L) and agar (15g/L) used to culture members of the Enterobacteriaceae family and for coliphage plaque assays.

Preparation Note

Recommended use at 30.5 g per liter.

Other Notes

Contains: Tryptone, yeast extract, sodium chloride and agar.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Chujin Ruan et al.
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The amount of bacterial diversity present on many surfaces is enormous; however, how these levels of diversity persist in the face of the purifying processes that occur as bacterial communities expand across space (referred to here as range expansion) remains
Xiaoyun Qiu et al.
Applied and environmental microbiology, 70(11), 6435-6443 (2004-11-06)
We systematically investigated the physiological response as well as DNA damage repair and damage tolerance in Shewanella oneidensis MR-1 following UVC, UVB, UVA, and solar light exposure. MR-1 showed the highest UVC sensitivity among Shewanella strains examined, with D37 and
Yu-Fan Chuang et al.
Frontiers in cell and developmental biology, 9, 667879-667879 (2021-06-29)
Specific changes in the genome have been accomplished by the revolutionary gene-editing tool known as clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) system. The advent of programmable RNA editing CRISPR/Cas nucleases has made this gene-editing tool safer and more
Daria Julkowska et al.
Journal of bacteriology, 187(1), 65-76 (2004-12-17)
The natural wild-type Bacillus subtilis strain 3610 swarms rapidly on the synthetic B medium in symmetrical concentric waves of branched dendritic patterns. In a comparison of the behavior of the laboratory strain 168 (trp) on different media with that of
Mario Losen et al.
Biotechnology progress, 20(4), 1062-1068 (2004-08-07)
Shake-flask cultures are widely used for screening of high producing strains. To select suitable strains for production scale, cultivation parameters should be applied that provide optimal growth conditions. A novel method of measuring respiratory activity in shake-flask cultures was employed

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