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L2254

Sigma-Aldrich

Lipoprotein Lipase from bovine milk

ammonium sulfate suspension, ≥2,000 units/mg protein (BCA)

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Synonym(s):
Diacylglycerol acylhydrolase, Diacylglycerol lipase
CAS Number:
9004-02-8
Enzyme Commission number:
3.1.1.34 (BRENDA, IUBMB)
EC Number:
232-669-1
MDL number:
MFCD00131523
NACRES:
NA.54

biological source

bovine milk

Quality Level

200

form

ammonium sulfate suspension

specific activity

≥2,000 units/mg protein (BCA)

storage temp.

2-8°C

General description

Lipoprotein Lipase (LPL) from bovine milk is a glycoprotein. It exists as a homodimer and comprises two N-linked oligosaccharides. It is heat-labile.

Application

Lipoprotein Lipase from bovine milk has been used:
  • as a supplement to test its effect on DiI (1,1′-dioctadecyl-3,3,3′-tetramethyl-indocarbocyanine perchlorate)- very-low-density lipoprotein (VLDL) uptake in breast cancer MDA-MB-231 cells
  • to treat human brain microvascular endothelial cells (HBMECs) for the lipolysis of triglyceride-rich lipoproteins (TGRL)
  • to test its effect on gene expression in normal human astrocytes

Biochem/physiol Actions

Lipoprotein Lipase (LPL) from bovine milk contributes to maximal lipolytic activity. It associates with casein micelle. LPL regulates triglyceride utilization and displays positional specificity. Lipases, in general, catalyzes the lipolysis of triglycerides especially at the fatty acid in sn-1 and sn-3 positions of the triglyceride.

Unit Definition

One unit will release 1.0 nmole of p-nitrophenol per min at pH 7.2 at 37 °C using p-nitrophenyl butyrate as substrate.

Physical form

Suspension in 3.8 M ammonium sulfate, 0.02 M Tris HCl, pH 8.0

Preparation Note

Affinity purified

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Lipoprotein lipase and lipolysis in milk
Deeth, Hilton C
International dairy journal, 16, 555-562 (2006)
Alexander V Sorokin et al.
JCI insight, 7(10) (2022-04-08)
BackgroundAlthough traditional lipid parameters and coronary imaging techniques are valuable for cardiovascular disease (CVD) risk prediction, better diagnostic tests are still needed.MethodsIn a prospective, observational study, 795 individuals had extensive cardiometabolic profiling, including emerging biomarkers, such as apolipoprotein E-containing HDL-cholesterol
Dean Oldham et al.
Frontiers in cardiovascular medicine, 9, 926631-926631 (2022-08-02)
Lipoprotein lipase (LPL) plays a crucial role in preventing dyslipidemia by hydrolyzing triglycerides (TGs) in packaged lipoproteins. Since hypertriglyceridemia (HTG) is a major risk factor for cardiovascular disease (CVD), the leading cause of death worldwide, methods that accurately quantify the
Hnin Hnin Aung et al.
Journal of lipid research, 57(6), 955-968 (2016-04-19)
Dysfunction of the cerebrovasculature plays an important role in vascular cognitive impairment (VCI). Lipotoxic injury of the systemic endothelium in response to hydrolyzed triglyceride-rich lipoproteins (TGRLs; TGRL lipolysis products) or a high-fat Western diet (WD) suggests similar mechanisms may be
Richard E Morton et al.
Journal of lipid research, 63(2), 100166-100166 (2022-01-13)
Apolipoprotein F (ApoF) modulates lipoprotein metabolism by selectively inhibiting cholesteryl ester transfer protein activity on LDL. This ApoF activity requires that it is bound to LDL. How hyperlipidemia alters total plasma ApoF and its binding to LDL are poorly understood.
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Protocols

LPL Activity Assay Protocol

Lipoprotein lipase (LPL) hydrolyzes triglycerides associated with VLDL.

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