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Merck
CN

L2785

Lectin from Dolichos biflorus (horse gram)

lyophilized powder

Synonym(s):

Dolichos biflorus agglutinin, DBA

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About This Item

NACRES:
NA.32
UNSPSC Code:
12352202
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Product Name

Lectin from Dolichos biflorus (horse gram), lyophilized powder

form

lyophilized powder

potency

≤20 μg per mL agglutination activity

impurities

salt, essentially free

storage temp.

−20°C

Quality Level

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Analysis Note

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group A1 erythrocytes after 1 hour incubation at 25 °C.

Biochem/physiol Actions

DBA has anti-A1 human blood group specificity, useful for distinguishing between A1 and A2 blood types. DBA has strong anti-Cad activity, and has an affinity for terminal N-acetyl-α-D-galactosaminyl residues. Conjugates are prepared from affinity purified lectin.

Preparation Note

Purified by affinity chromatography.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

动植物源性产品
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Ann Marie Hynes et al.
Proceedings of the National Academy of Sciences of the United States of America, 111(27), 9893-9898 (2014-06-21)
Nephronophthisis (NPHP) is the major cause of pediatric renal failure, yet the disease remains poorly understood, partly due to the lack of appropriate animal models. Joubert syndrome (JBTS) is an inherited ciliopathy giving rise to NPHP with cerebellar vermis aplasia

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