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L3126

Lipase from porcine pancreas

Name: Lipase from porcine pancreas
Brand: SIGMA

Type II, ≥125 units/mg protein (using olive oil (30 min incubation)), 30-90 units/mg protein (using triacetin)

Synonym(s):

Pancreatic lipase, PPL, Triacylglycerol acylhydrolase, Triacylglycerol lipase

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About This Item

CAS Number:

9001-62-1

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-619-9

EC Number:

3.1.1.3 (BRENDA, IUBMB)

MDL number:

MFCD00131509

Specific activity:

≥125 units/mg protein (using olive oil (30 min incubation)), 30-90 units/mg protein (using triacetin)

Biological source:

Porcine pancreas

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Properties

biological source

Porcine pancreas

Quality Segment

200

type

Type II

form

lyophilized (cake)

specific activity

≥125 units/mg protein (using olive oil (30 min incubation)), 30-90 units/mg protein (using triacetin)

composition

protein, ≥20%

storage temp.

2-8°C

InChI

1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;

InChI key

QWZUIMCIEOCSJF-CHHCPSLASA-N

Description

Application

Lipase from porcine pancreas has been used:

for the analysis of fatty acid at the 2-position of triglycerides.
to determine the indigestible fraction of plant source that is resistant to the action of digestive enzymes.
as a component of dissolution media to study the dissolution profiles of theophylline.

Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.

Biochem/physiol Actions

Lipases catalyze the hydrolysis of esters in aqueous solutions and the synthesis of esters in non-aqueous solutions. They also produce hydroxyl and carboxylic groups through the hydrolysis of ester linkages in poly(ethylene terephthalate).

Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.

Analysis Note

Crude (Steapsin). Contains amylase and protease activity.

Protein determined by biuret.

Other Notes

One unit will hydrolyze 1.0 microequivalent of fatty acid from triacetin in 1 hr at pH 7.4 at 37°C. (pH 7.7 is used with olive oil as substrate)

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Pictograms

GHS08

Signal Word

Danger

Hazard Codes

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)