LB Broth with agar (Luria low salt)

Luria′s low salt LB is a microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates. The addition of agar provides a solid medium for microbial growth.

LB Broth with agar (Luria low salt) has been used:

• for culturing E. coli DH10B cells.
• in the isolation of diazotrophic bacteria.
• for transformation of competent E. coli strain Stbl3 for cloning.

Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin^®.

Luria′s low salt LB powder with agar provides:

• Easy scale-up using larger package sizes
• A budget-friendly alternative to pre-poured plates
• Standard formulation

1. Suspend 30.5 g in 1 L of distilled water.
2. Heat to boiling while stirring to dissolve.
3. Autoclave for 15 minutes at 121°C.
4. Cool to 50°C prior to dispensing into petri dishes.

Zeocin is a registered trademark of Cayla Sarl