L3397
LB Broth (Luria low salt)
Powder microbial growth medium
Synonym(s):
Luria Broth (Miller’s Modification), Luria-Bertani medium (LB)
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About This Item
NACRES:
NA.85
UNSPSC Code:
41106200
grade
Molecular Biology
Quality Segment
sterility
non-sterile
form
powder
composition
NaCl, 0.5 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L
technique(s)
microbiological culture: suitable
pH
6.8-7.2(1.55% solution)
application(s)
food and beverages
microbiology
storage temp.
room temp
suitability
suitable for molecular biology, nonselective for Escherichia coli, nonselective for coliforms
General description
Luria Broth is a bacterial culture medium used for the cultivation of members of the Enterobacteriaceae especially E.coli, as well as for coliphage plaque assays. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a very low-salt formulation.
Application
LB Broth (Luria low salt) has been used as a liquid broth medium in the broth microdilution technique.
Features and Benefits
Luria′s low salt LB powder provides:
- Standard formulation
- A budget-friendly alternative to liquid
- Easy scale-up using larger package sizes
Preparation Note
1. Suspend 15.5 g in 1 L of distilled water.
2. Autoclave for 15 minutes at 121°C.
2. Autoclave for 15 minutes at 121°C.
Stir to suspend 15.5g powder in 1L water. Autoclave for 15 minutes at 121C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).
Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
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