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L3397

LB Broth (Luria low salt)

Name: LB Broth (Luria low salt)
Brand: SIGMA

Powder microbial growth medium

Synonym(s):

Luria Broth (Miller’s Modification), Luria-Bertani medium (LB)

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About This Item

NACRES:

NA.85

UNSPSC Code:

41106200

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grade

Molecular Biology

Quality Level

200

sterility

non-sterile

form

powder

composition

NaCl, 0.5 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L

technique(s)

microbiological culture: suitable

pH

6.8-7.2(1.55% solution)

application(s)

food and beverages
microbiology

storage temp.

room temp

suitability

suitable for molecular biology, nonselective for Escherichia coli, nonselective for coliforms

Related Categories

Industrial Microbiology

Microbial Culture Media

General description

Luria Broth is a bacterial culture medium used for the cultivation of members of the Enterobacteriaceae especially E.coli, as well as for coliphage plaque assays. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a very low-salt formulation.

Application

LB Broth (Luria low salt) has been used as a liquid broth medium in the broth microdilution technique.

Features and Benefits

Luria′s low salt LB powder provides:

Standard formulation
A budget-friendly alternative to liquid
Easy scale-up using larger package sizes

Preparation Note

1. Suspend 15.5 g in 1 L of distilled water.
2. Autoclave for 15 minutes at 121°C.

Stir to suspend 15.5g powder in 1L water. Autoclave for 15 minutes at 121C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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Using buoyant mass to measure the growth of single cells.

Michel Godin et al.

Nature methods, 7(5), 387-390 (2010-04-13)

We used a suspended microchannel resonator (SMR) combined with picoliter-scale microfluidic control to measure buoyant mass and determine the 'instantaneous' growth rates of individual cells. The SMR measures mass with femtogram precision, allowing rapid determination of the growth rate in

Impacts of Metabolism and Organic Acids on Cell Wall Composition and Pseudomonas aeruginosa Susceptibility to Membrane Active Antimicrobials.

Giorgia Manzo et al.

ACS infectious diseases, 7(8), 2310-2323 (2021-07-31)

Reliable antimicrobial susceptibility testing is essential in informing both clinical antibiotic therapy decisions and the development of new antibiotics. Mammalian cell culture media have been proposed as an alternative to bacteriological media, potentially representing some critical aspects of the infection

Difco Manual, 272-272 (1998)

Luria broth (LB) and Luria agar (LA) media and their uses protocol

MacWilliams MP and Liao MK

Journal of Separation Science (2006)

CD4+ and CD8+ Circulating Memory T Cells Are Crucial in the Protection Induced by Vaccination with Salmonella Typhi Porins.

Luis Ontiveros-Padilla et al.

Microorganisms, 9(4) (2021-05-01)

Salmonella enterica serovar Typhi (S. Typhi) porins, OmpC and OmpF, are potent inducers of the immune response against S. Typhi in mice and humans. Vaccination with porins induces the protection against 500 LD50 of S. Typhi, life-lasting bactericidal antibodies and

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Protocols

Microbial Growth Protocols

General protocols for growth of competent cells in microbial medium.

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Global Trade Item Number

SKU	GTIN
L3397-1KG	04061833951057
L3397-250G	04061833951064

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