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L3522

LB Broth (Miller)

Name: LB Broth (Miller)
Brand: SIGMA

Highly-referenced nutrient-rich microbial growth powder medium, suitable for regular E.coli culture

Synonym(s):

Luria Broth, Miller’s LB Broth

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About This Item

NACRES:

NA.85

UNSPSC Code:

41106200

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grade

Molecular Biology

Quality Level

200

description

Miller′s Modification, 10 g/L NaCl

sterility

non-sterile

form

powder

composition

NaCl, 10 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L

pH

6.8-7.2(2.5% solution)

application(s)

food and beverages

storage temp.

room temp

suitability

nonselective for Escherichia coli, nonselective for coliforms

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Related Categories

Cloning & Expression

Industrial Microbiology

Microbial Culture Media

Molecular Cloning & Protein Expression

General description

Miller′s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates.

Application

LB Broth (Miller) has been used as a culture medium for E coli, Pseudomonas putida KT2440, Vibrio parahaemolyticus, and soil samples for the isolation of Bacillus spp.
Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added.

Biochem/physiol Actions

Luria Broth is used for maintenance and propagation of Escherichia coli. The E. coli grow faster in Luria Broth because the tryptone and yeast supply essential growth factors that the E. coli would otherwise have to synthesize. Luria Broth also contains essential electrolytes for transport and osmotic balance, due to the NaCl component.

Features and Benefits

Miller′s LB powder provides:

Easy scale-up using larger package sizes
A budget-friendly alternative to liquid
Standard formulation

Preparation Note

1. Suspend 25 g in 1 L of distilled water.
2. Autoclave for 15 minutes at 121 °C.
To prepare the medium of Luria, Adams and Ting (also known as LC broth: Aseptically add 25 ml of sterile 0.1 M calcium chloride after autoclaving.

Stir to suspend 25g powder in 1L water. Autoclave for 15 minutes at 121C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

监管及禁止进口产品

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Use of a design of experiments approach to optimise production of a recombinant antibody fragment in the periplasm of Escherichia coli: selection of signal peptide and optimal growth conditions.

Ikhlaas M Kasli et al.

AMB Express, 9(1), 5-5 (2019-01-09)

Production of recombinant proteins such as antibody fragments in the periplasm of the bacterium Escherichia coli has a number of advantages, including the ability to form disulphide bonds, aiding correct folding, and the relative ease of release and subsequent capture

A Frameshift Mutation in wcaJ Associated with Phage Resistance in Klebsiella pneumoniae.

Demeng Tan et al.

Microorganisms, 8(3) (2020-03-12)

Phage therapy is a potential and promising avenue for controlling the emergence and spread of multidrug-resistant (MDR) Klebsiella pneumoniae, however, the rapid development of anti-phage resistance has been identified as an obstacle to the development of phage therapy. Little is

Selecting lipopeptide-producing, Fusarium-suppressing Bacillus spp.: Metabolomic and genomic probing of Bacillus velezensis NWUMFkBS10. 5

Adeniji A, et al.

MicrobiologyOpen, e742-e742 (2018)

Single-cell bacteria growth monitoring by automated DEP-facilitated image analysis

Peitz I and van Leeuwen R

Lab on a chip, 10(21), 2944-2951 (2010)

Kinetic understanding of nitrogen supply condition on biosynthesis of polyhydroxyalkanoate from benzoate by Pseudomonas putida KT2440

Xu Z, et al.

Bioresource Technology, 273, 538-544 (2019)

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Global Trade Item Number

SKU	GTIN
L3522-250G	04061835573479
L3522-1KG	04061835573462

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