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L3759

Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia)

Name: Lectin from <I>Bandeiraea simplicifolia</I> (<I>Griffonia simplicifolia</I>)
Brand: SIGMA

biotin conjugate, lyophilized powder

Synonym(s):

Bandeirea simplicifolia agglutinin, BS-I

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About This Item

NACRES:

NA.32

UNSPSC Code:

12352200

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Properties

conjugate

biotin conjugate

form

lyophilized powder

potency

<250 μg per mL agglutination activity (using human blood group A erythrocytes.)

composition

Protein, ~85% E1%/280

extent of labeling

~5 mol biotin per mol protein

storage temp.

2-8°C

Description

Application

Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia) has been used:

for highlighting murine endothelial cells
to identify fetal capillaries in endothelial immunohistochemical staining, conjugated with biotin
to detect tumor total vessels

Biochem/physiol Actions

BS-I has a major affinity for terminal α-D-galactosyl residues with a secondary affinity for terminal N-acetyl-α-D-galactosaminyl residues.

Physical form

Contains sodium citrate

Analysis Note

Agglutination activity is expressed in μg/mL and is determined from serial dilutions of a 1 mg/mL solution using phosphate buffered saline, pH 6.8, containing, for each lectin, calcium, magnesium, and manganese at different concentrations. This activity is the lowest concentration to agglutinate a 2% suspension of appropriate erythrocytes after 1 hr incubation at 25 °C.

Other Notes

BS-I is a tetrameric lectin consisting of two types of subunits designated A and B. There are five BS-I isolectins with different subunit composition: BSI-B₄, BSI-AB₃, BSI-A₂B₂, BSI-A₃B and BSI-A₄. BSI-B₄ is blood group B specific and has an exclusive affinity for terminal α-D-galactosyl residues, whereas BSI-A₄ has blood group A specificity and has a major affinity for terminal N-acetyl-α-D-galactosaminyl residues.

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Storage Class

13 - Non Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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