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L4146

Leptin human

Name: Leptin human
Brand: SIGMA

≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder

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About This Item

CAS Number:

177404-21-6

UNSPSC Code:

51111800

NACRES:

NA.32

MDL number:

MFCD01094205

Biological source:

Escherichia coli

Form:

lyophilized powder

Assay:

≥97% (SDS-PAGE)

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Properties

biological source

Escherichia coli

Quality Segment

100

recombinant

expressed in E. coli

sterility

sterile; 0.2 μm filtered

assay

≥97% (SDS-PAGE)

form

lyophilized powder

potency

≤3.0 ng/mL ED₅₀/EC₅₀

mol wt

predicted mol wt ~16 kDa

impurities

≤1.0 EU/μg endotoxin

UniProt accession no.

P41159

shipped in

ambient

storage temp.

−20°C

Gene Information

human ... LEP(3952)

Description

General description

The gene LEP (leptin) is mapped to human chromosome 7q31. It is a 16kDa peptide. Leptin is released in the circulation and goes to the CNS (central nervous system). In CNS, it works by associating with leptin receptor.

Application

Leptin human has been used:

to study the effect of leptin on ACAT1 (acyl-coenzyme A:cholesterol acyltransferase 1) expression and cholesterol efflux from primary cultured human macrophages
to study the effect of leptin on expression of IGFBP2 (insulin growth factor binding protein 2) in skeletal muscle
to study the effect of leptin on HLA-G (human leukocyte antigen G) expression in placental trophoblasts
to study the effect of leptin on in vitro maturation, fertilization and development of horse oocytes after ICSI (intracytoplasmic sperm injection)

Biochem/physiol Actions

Leptin is believed to play a role in normal pregnancy development.

Mutations in LEP (leptin) are linked with type-2 diabetes. Presence of high levels of leptin in plasma is related to adiposity.

Hormone produced primarily in adipocytes; primary site of action appears to be on neurons in the hypothalamus that are involved in regulating energy balance, appetite, and body weight.

Leptin is a hormone produced primarily in adipocytes, although leptin mRNA has also been identified in placenta and fetal tissues, gastric tissue and liver. Its primary site of action appears to be on neurons in the hypothalamus that are involved in regulating energy balance, appetite, and body weight. Leptin increases the production of nitric oxide in endothelial cells and stimulates angiogenesis in vitro and in vivo.
Human and mouse leptin share ~84% sequence identity.

Physical form

Lyophilized from a 0.2 μm filtered solution with 0.0045 mM NaHCO₃

Analysis Note

The biological activity is measured by its ability to induce proliferation of leptin-dependent human OB-R transfected murine BAF3 cells.

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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