L5263
Lyticase from Arthrobacter luteus
partially purified powder, ≥1,500 units/mg protein
form
partially purified powder
specific activity
≥1,500 units/mg protein
composition
Protein, ≥10% biuret
storage temp.
2-8°C
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Biochem/physiol Actions
Lyticase hydrolyzes poly-β(1→3)-glucose such as yeast cell wall glucan.
Physical form
Partially purified powder containing ammonium sulfate and stabilizer
Other Notes
One unit will produce a ΔA800 of 0.001 per min at pH 7.5 at 25 °C, using a suspension of yeast as substrate in a 3 mL reaction mixture.
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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Sanford J Silverman et al.
Proceedings of the National Academy of Sciences of the United States of America, 107(15), 6946-6951 (2010-03-26)
Oscillations in patterns of expression of a large fraction of yeast genes are associated with the "metabolic cycle," usually seen only in prestarved, continuous cultures of yeast. We used FISH of mRNA in individual cells to test the hypothesis that
Electrophoretic karyotype analysis. Pulsed field gel electrophoresis.
J Maule
Methods in molecular biology (Clifton, N.J.), 29, 221-252 (1994-01-01)
Yeast artificial chromosome recombinants in a global strategy for chromosome mapping. Amplification of internal and terminal fragments by PCR, and generation of fingerprints.
B Arveiler
Methods in molecular biology (Clifton, N.J.), 29, 403-423 (1994-01-01)
B N Doebbeling et al.
Clinical infectious diseases : an official publication of the Infectious Diseases Society of America, 16(3), 377-383 (1993-03-01)
Candida species are important nosocomial pathogens, particularly in immunocompromised and critically ill patients. A variety of methods have been used to differentiate strains, but an optimal system has not been established. We compared methods for typing a panel of nine
M A Pfaller et al.
Journal of clinical microbiology, 32(1), 59-64 (1994-01-01)
DNA subtyping by pulsed-field gel electrophoresis and in vitro susceptibility testing were used to study strain variation and fluconazole resistance in Candida albicans isolates from patients with AIDS undergoing azole (fluconazole and clotrimazole) therapy for oropharyngeal candidiasis. A total of
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