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Merck
CN

L5390

Lectin from Erythrina cristagalli (coral tree)

lyophilized powder

Synonym(s):

Erythrina cristagalli agglutinin, ECA

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32
MDL number:
Form:
lyophilized powder
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form

lyophilized powder

potency

<0.5 μg per mL agglutination activity

composition

Protein, ≥85% biuret

impurities

salt, free

storage temp.

−20°C

Quality Level

Biochem/physiol Actions

ECA is not blood group specific, but has an affinity for D-galactose and D-galactosides. It is reported to be mitogenic for human peripheral blood T lymphocytes.
Lectin or haemagglutinin is a sugar stereospecific binding protein or glycoprotein. It is capable of reversibly agglutinating cells. It is implicated in various cellular processes such as cell agglutination, homing of leukocytes, induction of mitosis in lymphocytes, interferon c (IFN c) and cytotoxicity. It is used as a blood typing reagent in immunohaematology. Lectin is also used as a reagent in testing erythrocyte polyagglutinable states. It is considered as a potential tool for analyzing cell surface architecture and related membrane functions.

Preparation Note

Purified by affinity chromatography.

Analysis Note

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of trypsinized human blood group O erythrocytes after 1 hr at 25 °C.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

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Human erythrocyte specific lectin from the seeds of Indian coral tree, Erythrina variegata Linn, var. orientali Linn, Merrill
Datta TK and Basu PS
Journal of Biosciences, 5(1), 25-30 (1983)
Purification, some properties of a D-galactose-binding leaf lectin from Erythrina indica and further characterization of seed lectin
Konozy EHE, et al.
Biochimie, 84(10), 1035-1043 (2002)

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