L5505
Lectin from Ulex europaeus (gorse, furze)
lyophilized powder
Synonym(s):
Ulex europaeus agglutinin, UEA-I
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About This Item
NACRES:
NA.32
UNSPSC Code:
12352202
Form:
lyophilized powder
form
lyophilized powder
Quality Segment
potency
<8 μg/mL agglutination activity
composition
Protein, ≥80% biuret
impurities
salt, essentially free
storage temp.
2-8°C
Biochem/physiol Actions
Ulex europaeus agglutinin has anti-H blood group specificity. There are two types of lectin: UEA I has an affinity for L -fucose and UEA II has an affinity for N,N′-diacetylchitobiose. The mol. wt. of UEA I was initially found to be 170,000 daltons. Later reports indicate that UEA I may form aggregates at neutral and basic pH and that the correct mol. wt. is 68,000 daltons.
Analysis Note
Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group O erythrocytes after 1 hour incubation at 25 °C.
Other Notes
Conjugates are prepared from affinity purified lectin.
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Storage Class
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Gloves, type N95 (US)
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