L6533
Lectin from Dolichos biflorus (horse gram)
biotin conjugate, buffered aqueous solution
Synonym(s):
Dolichos biflorus agglutinin, DBA
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About This Item
NACRES:
NA.32
UNSPSC Code:
12352202
Product Name
Lectin from Dolichos biflorus (horse gram), biotin conjugate, buffered aqueous solution
conjugate
biotin conjugate
form
buffered aqueous solution
composition
Protein, ≥2.5 mg/mL biuret
extent of labeling
≥3 mol biotin per mol lectin
storage temp.
−20°C
Quality Level
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Application
Lectin from Dolichos biflorus (horse gram) has been used:
- to characterize mouse uterine natural killer (uNK) cells
- in double staining with lectins to localize nuclear factor-κB (NF-κB) activation
- as a species-specific gonocyte marker for the determination of donor cell colonization
- to label undifferentiated spermatogonia/cat testicular tissue
Biochem/physiol Actions
DBA has anti-A1 human blood group specificity, useful for distinguishing between A1 and A2 blood types. DBA has strong anti-Cad activity, and has an affinity for terminal N-acetyl-α-D-galactosaminyl residues. Conjugates are prepared from affinity purified lectin.
Dolichos biflorus agglutinin (DBA) possesses binding activity for which it is used as a marker binucleate cells and can indicate the state of their differentiation. It serves as a label for primordial germ cells (PGCs), gonocytes, pre-spermatogonia and spermatogonia.
General description
Lectin from Dolichos biflorus (horse gram) is a heterotetrameric glycoprotein with a molecular mass of around 113 kDa. Dolichos biflorus agglutinin (DBA) is specific for terminal α-linked N-acetyl-D-galactosamine (GalNAc). It is a dietary lectin.
Packaging
Sold on the basis of mg active conjugate.
Physical form
contains 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM Ca2+
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Tubular NF-kB and AP-1 activation in human proteinuric renal disease
Mezzano SA, et al.
Kidney International, 60(4), 1366-1377 (2001)
Bovine sertoli cells colonize and form tubules in murine hosts following transplantation and grafting procedures
Zhang Z, et al.
Journal of Andrology, 29(4), 418-430 (2008)
Devendra Kumar Maurya et al.
Nature communications, 13(1), 6889-6889 (2022-11-14)
Stimulus transduction in cilia of olfactory sensory neurons is mediated by odorant receptors, Gαolf, adenylate cyclase-3, cyclic nucleotide-gated and chloride ion channels. Mechanisms regulating trafficking and localization of these proteins in the dendrite are unknown. By lectin/immunofluorescence staining and in
Effect of horse gram lectin (Dolichos biflorus agglutinin) on degranulation of mast cells and basophils of atopic subjects: Identification as an allergen
Pramod SN, et al.
International Immunopharmacology, 6(11), 1714-1722 (2006)
Spermatogonial stem cell organization in felid testis as revealed by Dolichos biflorus lectin
Escada-Rebelo S, et al.
Andrology, 4(6), 1159-1168 (2016)
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