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L7026

Sigma-Aldrich

Lithium chloride solution

8 M, for molecular biology, ≥99%

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Synonym(s):
LiCl
Linear Formula:
LiCl
CAS Number:
7447-41-8
Molecular Weight:
42.39
MDL number:
MFCD00011078
PubChem Substance ID:
24896448
NACRES:
NA.31

grade

for molecular biology

Quality Level

200

Assay

≥99%

concentration

8 M

anion traces

sulfate (SO42-): ≤0.01%

cation traces

Ba: ≤0.003%
Fe: ≤0.001%
K: ≤0.01%
Na: ≤0.20%
heavy metals (as Pb): ≤0.002%

foreign activity

DNase, RNase, protease, none detected

SMILES string

[Li+].[Cl-]

InChI

1S/ClH.Li/h1H;/q;+1/p-1

InChI key

KWGKDLIKAYFUFQ-UHFFFAOYSA-M

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Application

Lithium chloride solution has been used:
  • to obtain LiClPAM3 DCs form of dendritic cell
  • in LiCl buffer preparation for immunoprecipitation
  • in the preparation of wash buffer
  • in the preparation of radioimmunoprecipitation assay (RIPA) wash buffer

May be used to selectively precipitate RNA.

Biochem/physiol Actions

Lithium chloride has the ability to block glycogen synthase kinase (GSK). It may also possess anti-inflammatory effects at low and non-toxic concentrations.

Preparation Note

Prepared with 18 megaohm water
0.2 μm filtered

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H302 - H315 - H319

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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mTOR and neuroinflammation
Molecules to Medicine with mTOR, 317-329 (2016)
RNA Chromatin Immunoprecipitation (RNA-ChIP) in Caenorhabditis elegans
Cecere G and Grishok A
Nature Structural and Molecular Biology (2014)
Micro-chromatin Immunoprecipation (muChIP) Protocol for Real-time PCR Analysis of a Limited Amount of Cells
Gillotin S and Guillemot F
Bio-protocol, 6(12), e0159986-e0159986 (2016)
Allostimulatory Effects of Dendritic Cells with Characteristic Features of a Regulatory Phenotype
Kouwenberg M, et al.
PLoS ONE, 11(8), e0159986-e0159986 (2016)
Tong Ihn Lee et al.
Nature protocols, 1(2), 729-748 (2007-04-05)
Genome-wide location analysis, also known as ChIP-Chip, combines chromatin immunoprecipitation and DNA microarray analysis to identify protein-DNA interactions that occur in living cells. Protein-DNA interactions are captured in vivo by chemical crosslinking. Cell lysis, DNA fragmentation and immunoaffinity purification of
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