L7275
LB Broth (Lennox)
Tablet microbial growth medium
Synonym(s):
Lennox broth , Luria Bertani Broth
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About This Item
NACRES:
NA.85
UNSPSC Code:
12352200
grade
Molecular Biology
Quality Level
sterility
non-sterile
form
tablet
technique(s)
microbiological culture: suitable
application(s)
food and beverages
microbiology
storage temp.
room temp
suitability
nonselective for Escherichia coli, nonselective for coliforms
General description
Dehydrated Luria-Bertani (LB) broth is called the Lennox LB, which has amino acids as carbon source.
Lennox LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids and carbohydrates in a low-salt formulation.
Application
LB Broth (Lennox) has been used for culturing Escherichia coli and bacterial artificial chromosomes (BACs).
Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin®.
Features and Benefits
Lennox LB tablets provide:
- Convenient small package to eliminate weighing
- Fast preparation – no stirring required
Preparation Note
Add each 1.1g tablet in 48.3mL water (no stirring required). Autoclave for 20 minutes at 121C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).
Other Notes
10 g/L Tryptone
5 g/L Yeast Extract
5 g/L NaCl
2.2 g/L inert binding agents
5 g/L Yeast Extract
5 g/L NaCl
2.2 g/L inert binding agents
Legal Information
Zeocin is a registered trademark of Cayla Sarl
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
监管及禁止进口产品
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Protocols
General protocols for growth of competent cells in microbial medium.
在从小型培养到发酵级的不同规模下培养大肠杆菌。包括了用于启动起始培养物、悬浮细胞、单一培养物和平板噬菌体M13的实验方案。
Related Content
Instructions
Simultaneous quantification of active carbon-and nitrogen-fixing communities and estimation of fixation rates using fluorescence in situ hybridization and flow cytometry
McInnes AS, et al.
Applied and Environmental Microbiology, 80(21), 6750-6759 (2014)
Fluorescence in situ hybridization of ductal lavage samples identifies malignant phenotypes from cytologically normal cells in women with breast cancer
Adduci KM, et al.
Cancer Cytopathology, 111(3), 185-191 (2007)
Escherichia coli Physiology in Luria-Bertani Broth
Sezonov G, et al.
Journal of Bacteriology, 189(23), 8746-8746 (2007)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| L7275-100TAB | 04061833965450 |
| L7275-500TAB | 04061833965467 |