L8005
β-Lactoglobulin B from bovine milk
≥90% (PAGE)
Synonym(s):
β-LG, Bos d 5, beta-LG
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About This Item
Form:
powder
Assay:
≥90% (PAGE)
Biological source:
bovine milk
Mol wt:
18,276 Da by calculation
biological source
bovine milk
Quality Segment
assay
≥90% (PAGE)
form
powder
mol wt
18,276 Da by calculation
technique(s)
HPLC: suitable, electrophoresis: suitable
UniProt accession no.
storage temp.
2-8°C
Gene Information
bovine ... LGB(280838)
General description
β-Lactoglobulin B (β-LG B), a isoform of β-Lactoglobulin, is a small protein of 162 amino acids with a molecular mass of 18.2 kDa and optimum pH of 5.2. It is present in high level in casein and is relatively low in raw bovine milk. β-LG has eight-stranded β-barrel (strands A-H) succeeded by a three-turn α-helix and a final β-strand (strand I), that forms part of the dimerization interface.
Milk from dairy cows contains the protein β-lactoglobulin (BLG). It naturally occurs in a number of genetic variants, and the most prevalent bovine variants are known as BLG A and BLG B.
Application
β-Lactoglobulin B from bovine milk has been used as:
- a protein standard in SDS-polyacrylamide electrophoresis for quantification of milk protein fractions
- in the calibration of reversed phase- high-performance liquid chromatography (HPLC) for caseins quantification
- for immobilization on the biosensor surface and a calibration standards in biosensor assay
β-Lactoglobulin was used in the identification of the genetic variants of κ-casein in milk by isoelectric focusing electrophoresis.
Biochem/physiol Actions
β-Lactoglobulin B (β-LG B) show less inhibitory effect on the Staphylaococcus sp compared to β-LG A.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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