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L8258

Lectin from Wisteria floribunda

lyophilized powder

Synonym(s):

Wisteria floribunda agglutinin, WFA

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32
MDL number:
MFCD00166187
Form:
lyophilized powder
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Quality Segment

form

lyophilized powder

potency

<16 μg/mL agglutination activity

composition

Protein, ~95% E1%/280

storage temp.

−20°C

General description

Lectin is a carbohydrate-binding protein found in plant roots, rhizomes, leaves, seeds, and stems. Lectin is located in the cytoplasm as well as in the nucleus.

Application

Lectin from Wisteria floribunda has been used:
  • to study the perineuronal nets and parvalbumin nets in mouse brain by immunofluorescence
  • to evaluate the amount of N-acetylgalactosamine (GalNAc) transferred to Gaussia luciferase (GLuc) substrates by microplate assay
  • to study its binding effects on perineuronal nets

Biochem/physiol Actions

Lectin binds with high affinity to the glycans of polysaccharides, glycolipids, and glycoproteins. It is involved in plant defense.
WFA is not blood group specific, but has an affinity for N-acetyl-D-galactosamine.

Packaging

Package size based on protein content

Analysis Note

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group A erythrocytes after 1 hr incubation at 25 °C.

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Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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