L9267
Lectin from Lens culinaris (lentil)
lyophilized powder
Synonym(s):
Lens culinaris agglutinin, LcH
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About This Item
NACRES:
NA.32
UNSPSC Code:
12352202
Form:
lyophilized powder
form
lyophilized powder
potency
<16 μg per mL agglutination activity
impurities
salt, free
storage temp.
2-8°C
Quality Level
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General description
Lectins are proteins of plant origin, which have the ability to bind to certain neuronal surface glycoproteins and glycolipids. This ability of theirs helps them to be used as neuroanatomical tracers, as they are taken and transported in a retrograde or anterograde fashion.
Application
Lectin from Lens culinaris (lentil) is used for size- or charge-based separation of proteins with the ANA. (Anisotropic Nanofilter Array: ANA).
Biochem/physiol Actions
LcH is not blood group specific. It has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. LcH is comprised of two isomers, LcH-A (mol. wt. 49,000) and LcH-B (mol. wt. 49,000). LcH-A is mitogenic. Conjugates are prepared from purified lectin.
Preparation Note
Highly purified by affinity chromatography.
Analysis Note
Agglutination activity is expressed in μg per mL and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.
Contains two major bands on electrophoresis corresponding to isolectins LcH-A and LcH-B.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
常规特殊物品
低风险生物材料
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Anders Heebøll-Nielsen et al.
Biotechnology and bioengineering, 87(3), 311-323 (2004-07-29)
This work presents the development, testing, and application in high-gradient magnetic fishing of superparamagnetic supports for adsorption of lectins. Various approaches were examined to produce affinity, mixed mode, and hydrophobic charge induction type adsorbents. In clean monocomponent systems affinity supports
U K Nath et al.
TheScientificWorldJournal, 2014, 372405-372405 (2015-01-13)
Lentil is one of the most important pulse crops in the world as well as in Bangladesh. It is now considered a main component for training and body building practising in first world countries. Yield varies tremendously from year to
Jianping Fu et al.
Nature nanotechnology, 2(2), 121-128 (2008-07-26)
Microfabricated regular sieving structures hold great promise as an alternative to gels to improve the speed and resolution of biomolecule separation. In contrast to disordered porous gel networks, these regular structures also provide well defined environments ideal for the study
Oscar A Peralta et al.
Reproductive biology and endocrinology : RB&E, 11, 55-55 (2013-06-27)
The objectives of the study were to characterize the expression of the α- and β-subunits of granulocyte-macrophage colony stimulating factor (GM-CSF) receptor in bovine cumulus cells and oocytes and to determine the effect of exogenous GM-CSF on cumulus cells expansion
C R Gerfen et al.
Brain research, 290(2), 219-238 (1984-01-09)
A new neuroanatomical method for tracing connections in the central nervous system based on the anterograde axonal transport of the kidney bean lectin, Phaseolus vulgaris-leucoagglutinin (PHA-L) is described. The method, for which a detailed protocol is presented, offers several advantages
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