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L9420

Luciferase from Photinus pyralis (firefly)

recombinant, expressed in E. coli, buffered aqueous solution, ≥10×1010 units/mg protein

Synonym(s):

Luciferase firefly

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About This Item

CAS Number:
61970-00-1
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
1.13.12.7 (BRENDA, IUBMB)
MDL number:
MFCD00131546
Specific activity:
≥10×1010 units/mg protein
Assay:
≥98% (SDS-PAGE)
Recombinant:
expressed in E. coli
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recombinant

expressed in E. coli

Quality Level

200

assay

≥98% (SDS-PAGE)

form

buffered aqueous solution

specific activity

≥10×1010 units/mg protein

mol wt

62 kDa

shipped in

dry ice

storage temp.

−20°C

General description

Firefly luciferase catalyzes the reaction of luciferin with ATP and leads to the production of yellow-green light. The enzyme has a molecular weight of 62kDa and is expressed in peroxisomes. Luciferase is considered as a model to study protein–anesthetic interactions. Firefly luciferase is highly useful in cell biology and molecular biology, as a reporter of gene function and for the quantification of ATP.

Application

Luciferase from Photinus pyralis (firefly) has been used as a component of lysis solution to measure luminescence signals, as part of the study to determine chemical signals which can activate the karrikin insensitive 2 (KAI2) pathway.

Physical form

Supplied a a solution in 25mM Tris-acetate, pH 7.8 , 1mM EDTA, 1mM DTT, 0.2M ammonium sulfate, 15% glycerol and 30% ethylene glycol

Other Notes

One luciferase enzyme unit will produce one Relative Light Unit (RLU) at 20-25 °C over a 10 second period, measured in 100 μl assay mixture containing 40 pmol ATP and 15 nmol luciferin in Tris-glycine buffer, pH 7.6, using a GloMax 20/20 Luminometer.

Unit Definition Conversion Factor: There are approximately 9000 Relative Light Units (RLU) per one traditional Light Unit that uses a peak height equivalent to 0.02 μCi of 14C in a PPO/POPOP cocktail.

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pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Resp. Sens. 1 - STOT RE 2 Oral

target_organs

Kidney

Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

231.8 °F

flash_point_c

111 °C

Regulatory Information

常规特殊物品

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Certificates of Analysis (COA)

Lot/Batch Number
0000488205
0000488205
0000458776
0000458776
0000237830

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Articles

TokeOni: Properties and Applications

Bioluminescence imaging (BLI) systems allows for high-sensitive and noninvasive monitoring of cell proliferation, activity of signaling pathways and protein-protein interactions in living tissues.

Bioluminescent Firefly Luciferase Assays

Firefly luciferase is a sensitive reporter for gene studies due to its absence in mammalian cells or tissues.

TokeOni Analog - seMpai: Properties & Applications

seMpai substrate is suitable for near-infrared BLI in biological experiments, offering high solubility for extended bioluminescent applications.

Griffiths A J F, et al.
An Introduction to Genetic Analysis. (2000)
Crystal structure of firefly luciferase throws light on a superfamily of adenylate-forming enzymes.
Conti E, et al.
Structure, 4(3), 287-298 (1996)
Isabella Nymann Westensee et al.
Small (Weinheim an der Bergstrasse, Germany), 17(24), e2007959-e2007959 (2021-05-11)
Artificial cells (ACs) aim to mimic selected structural and functional features of mammalian cells. In this context, energy generation is an important challenge to be addressed when self-sustained systems are desired. Here, mitochondria isolated from HepG2 cells are employed as
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Global Trade Item Number

SKUGTIN
L9420-5X1MG04061833234877
L9420-1MG04061826703458