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L9530

Sigma-Aldrich

Linoleic Acid-Albumin from bovine serum albumin

liquid, sterile-filtered, BioReagent, suitable for cell culture

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Synonym(s):
Linoleic Acid-Albumin from BSA
NACRES:
NA.75

description

Contains 2 moles linoleic acid per mole BSA

Quality Level

300

sterility

sterile-filtered

product line

BioReagent

form

liquid

concentration

100 mg/mL BSA in DPBS
90-120 mg/mL protein (biuret)

technique(s)

cell culture | mammalian: suitable

impurities

≤400 EU/mL endotoxin

storage temp.

2-8°C

Related Categories

General description

Fatty Acid-Albumin - complexes have been employed as alternative sources of lipids in the development of serum-free media. Fatty acids bind to serum proteins in high proportions. Such proteins may release beneficial fatty acids and bind those that are inhibitory. Linoleic acid, a precursor of prostaglandins bound to BSA is beneficial to the growth of a variety of cell types (e.g., BHK, hybridoma). It also improves its stability and solubility in culture media.

Application

Linoleic Acid-Albumin from bovine serum albumin is used in the cell line culture and cell differentiation medium.
Linoleic Acid-Albumin from bovine serum albumin is used in the cell line culture and cell differentiation medium.

Other Notes

Recommended usage: 1 ml/100 ml medium

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

含少量动物源组分生物产品

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Chiu-Fang Liang et al.
IUBMB life, 54(5), 275-279 (2003-02-18)
Epidermal growth factor (EGF) was reported to regulate triacyl glycerol synthesis in various cells. Linoleic acid and its metabolites were thought to modulate the signal transduction of growth factors. This study determined whether linoleic acid regulated the effect of EGF
Dong-Hwan Kim et al.
Lipids, 55(2), 163-171 (2020-02-15)
The regulation of adipocyte differentiation is an important factor for production efficiency and meat quality in the poultry industry. The purpose of this study was to develop a new in vitro model of adipogenic differentiation of chicken embryonic fibroblasts (CEF).
Doreen Reichert et al.
Cells, 10(4) (2021-05-01)
Chronic liver diseases are associated with excessive deposition of extracellular matrix proteins. This so-called fibrosis can progress to cirrhosis and impair vital functions of the liver. We examined whether the receptor tyrosine kinase (RTK) class III inhibitor Crenolanib affects the
Cécile Coste et al.
PloS one, 12(7), e0177962-e0177962 (2017-07-07)
Adult neural crest stem-derived cells (NCSC) are of extraordinary high plasticity and promising candidates for use in regenerative medicine. Several locations such as skin, adipose tissue, dental pulp or bone marrow have been described in rodent, as sources of NCSC.
Robert J van Eijkeren et al.
Frontiers in endocrinology, 11, 479-479 (2020-08-28)
The complex direct and indirect interplay between adipocytes and various adipose tissue (AT)-resident immune cells plays an important role in maintaining local and whole-body insulin sensitivity. Adipocytes can directly interact with and activate AT-resident invariant natural killer T (iNKT) cells
View All Related Papers

Articles

Linoleic acid in Cell Culture

Importance and uses of linoleic acid in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures

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