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Merck
CN

LGC600511

E.Cloni 10G Elite (SOLOs) Electrocompetent Cells

Cfu/μg >2 x 1010, pkg of 12 reactions (12 x 25 µL)

Synonym(s):

Standard cloning competent cells, DNA cloning, Electrocompetent E.Coli, Electrocompetent cells, Electroporation, competent cells

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About This Item

UNSPSC Code:
41106202
NACRES:
NB.43
Biological source:
Escherichia coli
Growth mode:
growth or suspension
Manufacturer/tradename:
Lucigen 60051-1

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biological source

Escherichia coli

manufacturer/tradename

Lucigen 60051-1

packaging

pkg of 12 reactions (12 x 25 µL)

growth mode

growth or suspension

technique(s)

microbiological culture: suitable

cell transformation

competent cell type: electrocompetent
transformation efficiency: 2 x 1010 cfu/μg

shipped in

dry ice

storage temp.

-65 to -96°C

Related Categories

General description

E. cloni electrocompetent cells incorporate a unique manufacturing technology that increases transformation efficiency, recombinant yields and reliability. These cells provide solutions for a wide range of applications at economical prices.

Packaged as SOLOs (1 transformation per tube, 12 x 25µl)

Application

E. cloni 10G Competent Cells. Library construction, cloning, subcloning, and plasmid isolation with or without blue/white screening.

Features and Benefits

  • Optimized genetics for high yields: phage T1 resistant, endonuclease and recombination minus, blue/white screening-capable (without IPTG induction)
  • Supports cloning methylated DNA
  • Replace standard cloning strains (e.g., OP10, DH5α, DH10B, XL1-Blue, etc.)

Transformation efficiency: ≥ 2 x 1010 (cfu/µg pUC DNA)

Genotype: E.cloni 10G: F¯ mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 ɸ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697 galU galK rpsL (StrR) nupG λ- tonA

Components


  • E. cloni 10G Elite Electrocompetent Cells
  • pUC 19 Transformation Control
  • Recovery Media

Regulatory Information

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