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LGC600524

E.Cloni 10G Elite SixPacks Electrocompetent Cells

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pkg of 48 reactions (8 x 150 µL), Cfu/μg >2 x 1010

Synonym(s):

High Efficiency competent cells

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About This Item

UNSPSC Code:
41106202

Key Documents

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biological source

Escherichia coli

manufacturer/tradename

Lucigen 60052-4

packaging

pkg of 48 reactions (8 x 150 µL)

growth mode

growth or suspension

technique(s)

microbiological culture: suitable

cell transformation

competent cell type: electrocompetent
transformation efficiency: 2 x 1010 cfu/μg

shipped in

dry ice

storage temp.

-65 to -96°C

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General description

E. cloni electrocompetent cells incorporate a unique manufacturing technology that increases transformation efficiency, recombinant yields and reliability. These cells provide solutions for a wide range of applications at economical prices.

Application

E. cloni 10G Competent Cells. Library construction, cloning, subcloning, and plasmid isolation with or without blue/white screening.

Features and Benefits

  • Optimized genetics for high yields: phage T1 resistant, endonuclease and recombination minus, blue/white screening-capable (without IPTG induction)
  • Supports cloning methylated DNA
  • Replace standard cloning strains (e.g., OP10, DH5α, DH10B, XL1-Blue, etc.)

Transformation efficiency: ≥ 2 x 1010 (cfu/µg pUC DNA)

Genotype: E. cloni 10G: F¯ mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 ɸ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697 galU galK rpsL (StrR) nupG λ- tonA

Components:


E. cloni 10G Elite Electrocompetent Cells


pUC 19 Transformation Control


Recovery Media

Regulatory Information

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