Skip to Content
Merck
CN

LGC600802

E.Cloni 10G SUPREME (DUOs) Electrocompetent Cells

pkg of 24 reactions (12 x 50 µL), Cfu/μg >4 x 1010

Synonym(s):

Competent Cells, DNA transformation, Electrocompetent E.Coli, Electroporation

Sign Into View Organizational & Contract Pricing

Select a Size

About This Item

UNSPSC Code:
41106202

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

biological source

Escherichia coli

manufacturer/tradename

Lucigen 60080-2

packaging

pkg of 24 reactions (12 x 50 µL)

growth mode

growth or suspension

technique(s)

microbiological culture: suitable

cell transformation

competent cell type: electrocompetent
transformation efficiency: 4 x 1010 cfu/μg

shipped in

dry ice

storage temp.

-65 to -96°C

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

E. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5α&trade, DH10B&trade, JM109, TOP10, etc. and directly replace them in cloning protocols. However, E. cloni electrocompetent cells incorporate a unique manufacturing technology that increases transformation efficiency, recombinant yields and reliability while decreasing costs. E.Cloni 10G Supreme Electrocompetent Cells have the highest transformation efficiency available from any supplier. Choose SUPREME Cells for the most demanding cloning situations, such as construction of large, high complexity libraries or cloning difficult targets, which require the greatest number of transformants possible.

Application

Clone, construct libraries, transform Gibson Assembly reactions all with high-yield, T1 phage-resistant cells in high efficiencies for routine and demanding clonings.

Features and Benefits

  • Optimized genetics for high yields: phage T1 resistant, endonuclease and recombination minus, blue/white screening-capable (without IPTG induction)
  • Supports cloning methylated DNA, BAC and Cosmid Cloning
  • Replace standard cloning strains (e.g., OP10, DH5α, DH10B, XL1-Blue, etc.)
Transformation efficiency: ≥ 4 x 1010 (cfu/µg pUC DNA)

Genotype: E. cloni 10G: F¯ mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 ɸ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697 galU galK rpsL (StrR) nupG λ- tonA

Components:


E. cloni 10G SUPREME Electrocompetent Cells


pUC 19 Transformation Control


Recovery Media

Regulatory Information

新产品
This item has

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service