E.Cloni 10G SUPREME (DUOs) Electrocompetent Cells

E. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5α&trade, DH10B&trade, JM109, TOP10, etc. and directly replace them in cloning protocols. However, E. cloni electrocompetent cells incorporate a unique manufacturing technology that increases transformation efficiency, recombinant yields and reliability while decreasing costs. E.Cloni 10G Supreme Electrocompetent Cells have the highest transformation efficiency available from any supplier. Choose SUPREME Cells for the most demanding cloning situations, such as construction of large, high complexity libraries or cloning difficult targets, which require the greatest number of transformants possible.

Clone, construct libraries, transform Gibson Assembly reactions all with high-yield, T1 phage-resistant cells in high efficiencies for routine and demanding clonings.

• Optimized genetics for high yields: phage T1 resistant, endonuclease and recombination minus, blue/white screening-capable (without IPTG induction)
• Supports cloning methylated DNA, BAC and Cosmid Cloning
• Replace standard cloning strains (e.g., OP10, DH5α, DH10B, XL1-Blue, etc.)

Transformation efficiency: ≥ 4 x 10¹⁰ (cfu/µg pUC DNA)

Genotype: E. cloni 10G: F¯ mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 ɸ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697 galU galK rpsL (StrR) nupG λ- tonA

Components:


E. cloni 10G SUPREME Electrocompetent Cells


pUC 19 Transformation Control


Recovery Media