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UNSPSC Code:
41106202
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biological source

Escherichia coli

manufacturer/tradename

Lucigen 60242-2

packaging

pkg of 24 reactions (12 x 80 µL)

growth mode

growth or suspension

technique(s)

microbiological culture: suitable

cell transformation

competent cell type: electrocompetent
transformation efficiency: 1 x 1010 cfu/μg

shipped in

dry ice

storage temp.

-65 to -96°C

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General description

Endura Competent Cells are a commonly used strain for cloning sequences that suffer unwanted recombination events in other strains. Clones with inverted repeats or other sequences prone to recombination are commonly found in retroviral genes, and require cells such as Endura to be propagated stably.

Application

  • Cloning and maintaining unstable or repetitive DNA sequences.
  • Recommended for high efficiency propagation of GeCKO CRISPR libraries (1)
  • Ideal for single-guide RNA lentiviral library construction (2)

Features and Benefits

  • Clone repetitive sequences and lentiviral libraries
  • Stabilize direct repeats and create lentiviral constructs

Transformation efficiency: ≥ 1 x 1010 (cfu/µg)

Genotype: Endura: recA13 supE44 ara-14 galK2 lacY1 proA2 rpsL20(StrR) xyl-5 λ– leu mtl-1 F– mcrB mrr hsdS20(rB–, mB–)

Components:


Endura Electrocompetent Cells


pUC 19 Trasformation Control


Recovery Media

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