生物来源
Escherichia coli
制造商/商品名称
Lucigen 60242-2
包装
pkg of 24 reactions (12 x 80 µL)
生长模式
growth or suspension
技术
microbiological culture: suitable
细胞转化
competent cell type: electrocompetent
transformation efficiency: 1 x 1010 cfu/μg
运输
dry ice
储存温度
-65 to -96°C
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一般描述
Endura Competent Cells are a commonly used strain for cloning sequences that suffer unwanted recombination events in other strains. Clones with inverted repeats or other sequences prone to recombination are commonly found in retroviral genes, and require cells such as Endura to be propagated stably.
应用
- Cloning and maintaining unstable or repetitive DNA sequences.
- Recommended for high efficiency propagation of GeCKO CRISPR libraries (1)
- Ideal for single-guide RNA lentiviral library construction (2)
特点和优势
- Clone repetitive sequences and lentiviral libraries
- Stabilize direct repeats and create lentiviral constructs
Transformation efficiency: ≥ 1 x 1010 (cfu/µg)
Genotype: Endura: recA13 supE44 ara-14 galK2 lacY1 proA2 rpsL20(StrR) xyl-5 λ– leu mtl-1 F– mcrB mrr hsdS20(rB–, mB–)
Components:
Endura Electrocompetent Cells
pUC 19 Trasformation Control
Recovery Media
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