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UNSPSC Code:
41106202
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biological source

Escherichia coli

manufacturer/tradename

Lucigen 60300-2

packaging

pkg of 24 reactions (12 x 50 µL)

growth mode

growth or suspension

technique(s)

microbiological culture: suitable

cell transformation

competent cell type: electrocompetent
transformation efficiency: 5×109 cfu/μg

shipped in

dry ice

storage temp.

-65 to -96°C

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General description

E. cloni EXPRESS BL21(DE3) Electrocompetent Cells offer high efficiency cloning and high level protein expression in the same cell. Cloning efficiencies are increased 25-1,000 fold relative to competitors’ preparations of BL21 cells, which is essential for construction of complex expression libraries. The unprecedented transformation efficiency of E. cloni EXPRESS Electrocompetent Cells (> 5 × 109 cfu/µg) eliminates the need for plasmid transfer from the cloning strain to the expression strain, saving days of work in a typical cloning and expression experiment.

Application

Cloning, routine protein expression

Features and Benefits

  • Routine protein expression
  • High transformation efficiency
  • Short protein expression protocol time

Transformation efficiency: ≥ 5 x 109 (cfu/µg)

Genotype: F – ompT hsdSB (rB- mB-) gal dcm (DE3)

Components:


E.Cloni EXPRESS BL21 (DE3) Electrocompetent Cells


pUC 19 Trasformation Control


Recovery Media

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