E. cloni EXPRESS BL21(DE3) Electrocompetent Cells offer high efficiency cloning and high level protein expression in the same cell. Cloning efficiencies are increased 25-1,000 fold relative to competitors’ preparations of BL21 cells, which is essential for construction of complex expression libraries. The unprecedented transformation efficiency of E. cloni EXPRESS Electrocompetent Cells (> 5 × 10⁹ cfu/µg) eliminates the need for plasmid transfer from the cloning strain to the expression strain, saving days of work in a typical cloning and expression experiment.

Cloning, routine protein expression

• Routine protein expression
• High transformation efficiency
• Short protein expression protocol time

Transformation efficiency: ≥ 5 x 10⁹ (cfu/µg)

Genotype: F – ompT hsdSB (rB- mB-) gal dcm (DE3)

Components:


E.Cloni EXPRESS BL21 (DE3) Electrocompetent Cells


pUC 19 Trasformation Control


Recovery Media