biological source
Escherichia coli
manufacturer/tradename
Lucigen 60300-2
packaging
pkg of 24 reactions (12 x 50 µL)
growth mode
growth or suspension
technique(s)
microbiological culture: suitable
cell transformation
competent cell type: electrocompetent
transformation efficiency: 5×109 cfu/μg
shipped in
dry ice
storage temp.
-65 to -96°C
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General description
E. cloni EXPRESS BL21(DE3) Electrocompetent Cells offer high efficiency cloning and high level protein expression in the same cell. Cloning efficiencies are increased 25-1,000 fold relative to competitors’ preparations of BL21 cells, which is essential for construction of complex expression libraries. The unprecedented transformation efficiency of E. cloni EXPRESS Electrocompetent Cells (> 5 × 109 cfu/µg) eliminates the need for plasmid transfer from the cloning strain to the expression strain, saving days of work in a typical cloning and expression experiment.
Application
Cloning, routine protein expression
Features and Benefits
- Routine protein expression
- High transformation efficiency
- Short protein expression protocol time
Transformation efficiency: ≥ 5 x 109 (cfu/µg)
Genotype: F – ompT hsdSB (rB- mB-) gal dcm (DE3)
Components:
E.Cloni EXPRESS BL21 (DE3) Electrocompetent Cells
pUC 19 Trasformation Control
Recovery Media
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