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LGCEC10010

TransforMax EC100 Electrocompetent E. coli

pkg of 20 reactions (10 x 100 µL), Cfu/μg ≥ 1 x 1010

Synonym(s):

Competent Cells, DNA transformation, Electrocompetent E.Coli, Electrocompetent cells, Electroporation

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About This Item

UNSPSC Code:
41106202
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biological source

Escherichia coli

manufacturer/tradename

Lucigen EC10010

packaging

pkg of 20 reactions (10 x 100 µL)

growth mode

growth or suspension

technique(s)

microbiological culture: suitable

cell transformation

competent cell type: electrocompetent
transformation efficiency: 1 x 1010 cfu/μg

shipped in

dry ice

storage temp.

-65 to -96°C

General description

The highly versatile TransforMax EC100 E. coli competent cells are ideal for most cloning applications. The cells provide very high transformation efficiency when tested against a wide range of supercoiled DNAs as well as DNA directly from a ligation reaction

Application

Routine cloning of DNA up to 200kb

Features and Benefits

  • High transformation efficiency with clones of all sizes, including BAC clones
  • lacZΔM15 for blue/white screening of recombinants
  • Restriction minus [mcrA, Δ(mrr-hsdRMS-mcrBC)] enables efficient cloning of methylated DNA
  • Recombination minus (recA1) for greater stability of large cloned inserts
  • Endonuclease minus (endA1) to ensure high yields of DNA (LGCC400EL10) for demanding applications such as library generation

Transformation efficiency: ≥ 1 x 1010 (cfu/µg pUC DNA)

Genotype :TransformMac EC100: F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL (StrR) nupG

Components:


TransforMax EC100 Electrocompetent E. coli


pUC 19 Trasformation Control


CopyControlTM Induction Solution

Regulatory Information

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