LGCEC10010
TransforMax EC100 Electrocompetent E. coli
pkg of 20 reactions (10 x 100 µL), Cfu/μg ≥ 1 x 1010
别名:
Competent Cells, DNA transformation, Electrocompetent E.Coli, Electrocompetent cells, Electroporation
生物来源
Escherichia coli
制造商/商品名称
Lucigen EC10010
包装
pkg of 20 reactions (10 x 100 µL)
生长模式
growth or suspension
技术
microbiological culture: suitable
细胞转化
competent cell type: electrocompetent
transformation efficiency: 1 x 1010 cfu/μg
运输
dry ice
储存温度
-65 to -96°C
一般描述
The highly versatile TransforMax EC100 E. coli competent cells are ideal for most cloning applications. The cells provide very high transformation efficiency when tested against a wide range of supercoiled DNAs as well as DNA directly from a ligation reaction
应用
Routine cloning of DNA up to 200kb
特点和优势
- High transformation efficiency with clones of all sizes, including BAC clones
- lacZΔM15 for blue/white screening of recombinants
- Restriction minus [mcrA, Δ(mrr-hsdRMS-mcrBC)] enables efficient cloning of methylated DNA
- Recombination minus (recA1) for greater stability of large cloned inserts
- Endonuclease minus (endA1) to ensure high yields of DNA (LGCC400EL10) for demanding applications such as library generation
Transformation efficiency: ≥ 1 x 1010 (cfu/µg pUC DNA)
Genotype :TransformMac EC100: F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL (StrR) nupG
Components:
TransforMax EC100 Electrocompetent E. coli
pUC 19 Trasformation Control
CopyControlTM Induction Solution
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