biological source
Escherichia coli
manufacturer/tradename
Lucigen EC10010
packaging
pkg of 20 reactions (10 x 100 µL)
growth mode
growth or suspension
technique(s)
microbiological culture: suitable
cell transformation
competent cell type: electrocompetent
transformation efficiency: 1 x 1010 cfu/μg
shipped in
dry ice
storage temp.
-65 to -96°C
General description
The highly versatile TransforMax EC100 E. coli competent cells are ideal for most cloning applications. The cells provide very high transformation efficiency when tested against a wide range of supercoiled DNAs as well as DNA directly from a ligation reaction
Application
Routine cloning of DNA up to 200kb
Features and Benefits
- High transformation efficiency with clones of all sizes, including BAC clones
- lacZΔM15 for blue/white screening of recombinants
- Restriction minus [mcrA, Δ(mrr-hsdRMS-mcrBC)] enables efficient cloning of methylated DNA
- Recombination minus (recA1) for greater stability of large cloned inserts
- Endonuclease minus (endA1) to ensure high yields of DNA (LGCC400EL10) for demanding applications such as library generation
Transformation efficiency: ≥ 1 x 1010 (cfu/µg pUC DNA)
Genotype :TransformMac EC100: F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL (StrR) nupG
Components:
TransforMax EC100 Electrocompetent E. coli
pUC 19 Trasformation Control
存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
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