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UNSPSC Code:
41105500
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packaging

pkg of 50 mL
pkg of 500 reactions

manufacturer/tradename

Lucigen QEF81050

technique(s)

DNA extraction: suitable

General description

The analysis of nucleic acid from formalin-fixed, paraffin-embedded (FFPE) specimens is challenging due to the extensive cross-linking of all tissue components during the fixation process. These challenges include chemical modification of the DNA, cross-linking of DNA with other molecules, degradation of the DNA, and the limited amount of nucleic acid in the samples.

The QuickExtract FFPE DNA Extraction Kit is a fast, simple, and inexpensive method for preparing genomic DNA for PCR amplification from archival samples. The protocol requires only heat treatment to melt the paraffin, lyse the cells, decrease the fomalin-induced cross-linking in the sample, and degrade compounds inhibitory to amplification. Following heat treatment, the sample DNA is ready for PCR

Application

Isolation of DNA from FFPE samples for PCR-based analysis, e.g., microsatellite detection, SNP detection, tumour heterogeneity studies, copy number detection, methylation analysis, and Short Tandem Repeat (STR) analysis

Features and Benefits

  • Simple, rapid extraction of PCR-ready DNA from challenging FFPE samples
  • Fast: PCR-ready DNA in minutes, not days
  • Simple: No spin columns or transfer steps which helps increase yields
  • Compatible: Extracted DNA is compatible with both real-time and end-point PCR
  • Safe: No xylene or phenol extractions used

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