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LH20100

Sephadex® LH-20 Size Exclusion Resin

140-550 mesh

Synonym(s):

Lipophilic Sephadex®

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About This Item

UNSPSC Code:
13111000
NACRES:
NB.21
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Product Name

Sephadex® LH-20,

form

slurry

Quality Level

technique(s)

LPLC: suitable

matrix

Agarose

matrix active group

polymer

swelling

1 g swells to 4 mL (water, methanol)

bead size

25-100 μm

operating pH range

2-13

separation technique

size exclusion (SEC)

storage temp.

room temp

Application

Sephadex® LH-20 is a crosslinked dextran-based resin used with organic solvent mobile phases for gel permeation, normal phase partition, and adsorption chromatography of lipids, steroids, fatty acids, hormones, vitamins, etc. It is also used in gel filtration chromatography, protein chromatography, gel filtration media, resins and separation media.
Sephadex® LH-20 has been used to isolate bioactive polyphenolic content from blackberry genotypes and tryptophan from the aqueous extract of lotus rhizomes.

Legal Information

Sephadex is a registered trademark of Cytiva


Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Protocols

Separation using Sephadex LH-20 column for Size Exclusion Chromatography media in polysaccharide network from Cytiva.


P Kolodziejczyk et al.
Free radical biology & medicine, 5(1), 13-25 (1988-01-01)
Ambient temperature incubation of the anticancer agent mitoxantrone with horseradish peroxidase and hydrogen peroxide converts it into a hexahydronaphtho[2,3-f]quinoxaline-7,12-dione in which one side chain has cyclized to the chromophore. The structure of this cyclic metabolite was secured by independent synthesis.
Beatriz Pereira Moreno et al.
Chemistry & biodiversity, 17(3), e1900694-e1900694 (2020-02-06)
Studies of the phytotoxic effects between plants can be a crucial tool in the discovery of innovative compounds with herbicide potential. In this sense, we can highlight ruzigrass (Urochloa ruziziensis), which is traditionally used in the crop rotation system in
A K Tandon et al.
Cancer research, 46(7), 3375-3377 (1986-07-01)
We described recently a modified charcoal-gelatin (MCG) assay for measuring progesterone receptor activity in low-protein cytosols. We showed that pre-mixing of gelatin with sample cytosols (final gelatin concentration 0.1%) and removal of unbound steroid by a 1% charcoal suspension with



Global Trade Item Number

SKUGTIN
LH20100-50G04061838551986
LH20100-100G04061838551962
LH20100-500G04061838551979
LH20100-6X100G04061838551993