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Merck
CN

LH20100

Sephadex® LH-20 Size Exclusion Resin

140-550 mesh

Synonym(s):

Lipophilic Sephadex®

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About This Item

UNSPSC Code:
13111000
NACRES:
NB.21
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Product Name

Sephadex® LH-20,

form

slurry

Quality Level

technique(s)

LPLC: suitable

matrix

Agarose

matrix active group

polymer

swelling

1 g swells to 4 mL (water, methanol)

bead size

25-100 μm

operating pH range

2-13

separation technique

size exclusion (SEC)

storage temp.

room temp

Application

Sephadex® LH-20 is a crosslinked dextran-based resin used with organic solvent mobile phases for gel permeation, normal phase partition, and adsorption chromatography of lipids, steroids, fatty acids, hormones, vitamins, etc. It is also used in gel filtration chromatography, protein chromatography, gel filtration media, resins and separation media.
Sephadex® LH-20 has been used to isolate bioactive polyphenolic content from blackberry genotypes and tryptophan from the aqueous extract of lotus rhizomes.

Legal Information

Sephadex is a registered trademark of Cytiva


Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Protocols

Separation using Sephadex LH-20 column for Size Exclusion Chromatography media in polysaccharide network from Cytiva.


S K Kundu et al.
Journal of lipid research, 20(7), 825-833 (1979-09-01)
Aminopropyl silica gel was prepared from porous silica gel and was used as a solid support for immunoadsorbent in the purification of anti-glycolipid antibodies. For neutral glycosphingolipids, a carboxyl function was generated by oxidation of the olefinic double bond of
Yizhong Cai et al.
Journal of chromatographic science, 43(9), 454-460 (2005-10-11)
HPLC characterization of reversed-phase (RP) high-performance liquid chromatography (HPLC) has been widely used in separation and identification of plant pigments. An effective RP-HPLC-based method is established to systematically isolate, identify, and quantitate the betalain pigments in the plants of 37
Suman B Sharma et al.
Journal of medicinal food, 14(4), 353-359 (2011-03-05)
Diabetes is accompanied by lipid abnormalities, which contribute significantly to cardiovascular morbidity and mortality in diabetic patients. We previously demonstrated the potent antihyperglycemic activity of the active principle (fraction II from Sephadex LH 20 chromatography [LH II]) isolated from ethanolic



Global Trade Item Number

SKUGTIN
LH20100-50G04061838551986
LH20100-100G04061838551962
LH20100-500G04061838551979
LH20100-6X100G04061838551993