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LVCAS9BST

Sigma-Aldrich

CAS9 Blasticidin Lenti Particles

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NACRES:
NA.51

packaging

vial of 200 μL

Quality Level

concentration

≥1 x 10 6 VP/ml virus (via p24 assay)

selection

blasticidin

shipped in

dry ice

storage temp.

−70°C

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General description

Ready-to-use Cas9 lentiviral particles are ideal for transducing a wide range of cell lines. The Cas9 Blasticidin Lentiviral particles efficiently integrate Cas9 into the genomes of both dividing and non-dividing cell lines for use with CRISPR genome editing. Stably integrate Cas9 and Blast using the convenient, ready-to-use lentiviral particles while avoiding the tedious lentivirus production process.

Application

Functional Genomics/Screening /Target Validation/Genome Editing

Components

200 ul of purified lentiviral particles (minimum of 1 X 106 TU/ml via a p24 assay) expressing Cas9 only with blasticidin resistance.

Linkage

Click here to order gRNA plasmid and virus.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

高风险级别生物产品--病毒,疫苗等

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Articles

Genome-wide loss-of-function screening is a powerful approach to discover genes and pathways that underlie biological processes. Now complete knockout is achievable with two optimized gRNAs per gene. Minimized clone number ensures the most specific screening possible while controlling time and cost.

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