CRISPR和基因编辑
CRISPR CAS9 蛋白的基本要素和服务的最佳组合
基因组工程的进步使研究人员能够实现前所未有的突破，从治愈遗传疾病到开发抗病作物。我们可靠的 CRISPR Cas9 蛋白必需品和工具为基于 CRISPR 的基因编辑研究从台式到更大规模的每一步提供全面支持。使用锌指核酸酶靶向单个基因。从两种不同的 PURedit® Cas9 胞嘧啶碱基编辑器试剂盒中选择用于 C 到 T 替换的试剂盒，并根据独特的编辑窗口进行优化，以实现具有挑战性的编辑。
从 CRISPR Cas9 蛋白到合成向导 RNA，再到用于 CRISPR 筛选的慢病毒产品，我们为您基于 CRISPR 的基因编辑研究的每一步都提供了终极产品套件。
西格玛-奥德里奇®高级基因组学产品组合提供终极基因组工程工具和服务套件，以释放您的研究潜能。无论您是要敲除、敲入、敲除还是过表达您的目标，我们全面的 CRISPR 基因编辑工具和服务套件都将使您的研究超越工作台。
产品
热门过滤器
Products
了解 PURedit® Cas9 胱氨酸碱基编辑器试剂盒如何立即提供正确的编辑。
Cas类最佳9种蛋白质，包括PURedit® Cas9 胞嘧啶碱基编辑蛋白试剂盒
Sigma-Aldrich® 以多种形式（质粒、慢病毒、冻干蛋白）提供多种 Cas9 变体（野生型、特异性增强型、镍酶、GFP 融合型、催化不活跃型）。我们最新推出的 PURedit® Cas9 胞嘧啶碱基编辑蛋白试剂盒，可以用我们的专家设计的两种酶进行确定的胞嘧啶碱基编辑，方便 RNP 的递送，专为成功而设计。
Guaranteed CRISPR gRNA
Every SygRNA® ；合成的单导 RNA (sgRNA)、crRNA 和 tracrRNA 都是采用我们专有的工艺和试剂，用现有最高质量的材料合成的。无论您的目标、细胞类型或应用如何，我们都能确保编辑活性，并提供业内最全面的担保。利用我们的免费定制 CRISPR 设计工具应用程序，您可以为所有类型的 CRISPR 应用设计自己的引导程序，包括使用我们的 PURedit® Cas9 精确和灵活胞嘧啶碱基编辑器进行胞嘧啶碱基编辑。
CRISPR抑制和激活（CRISPRi和CRISPRa）
使用 Sigma-Aldrich® CRISPR 激活和 CRISPR 抑制文库进行基因敲除和过表达实验，更深入地了解复杂的生物网络。CRISPRi 和 SAM CRISPRa 慢病毒库可现成提供，也可根据您的具体需求定制。
具有 10x Genomics 兼容性的 CRISPR 筛选
Sigma-Aldrich® 为 CRISPR 单细胞筛选提供唯一具有 10x Genomics 认证的兼容慢病毒池。我们的优化定制慢病毒载体利用特征条码技术与 Chromium 单细胞基因表达 v3 和 v3.1 (Next GEM) 解决方案完全兼容。
相关资源
- Brochure: Molecular Essentials Guide
Learn how our comprehensive molecular tools and reagents provide precise, ready-to-use solutions for your workflows.
- Article: CRISPR Cas9 Proteins
超丰富的优质 Cas9 蛋白组合，确保您始终可找到满足自身特定实验需求的完美 CRISPR 试剂。严苛纯化、NLS 信号、增强特异性和活性，确保您高效、简便地开展基因编辑实验。
- Article: CRISPR Cas9 Protein Product Selection Guide
A convenient CRISPR Cas9 protein selection guide to assist with your CRISPR RNP, lentivirus, mRNA, plasmids, vectors and control needs.
- CRISPR使用许可协议
This Product and its use are the subject of one or more of the following patents controlled by The Broad Institute, Inc. (BROAD), the Massachusetts Institute of Technology (MIT), or the President and Fellows of Harvard College (HARVARD): U.S. Patent Nos. 8,697,357; 8,771,945, and any substitutions, divisions, continuations, reissues, renewals, re-examinations or extensions, and corresponding foreign patent applications and patents.
- Article: Cas9-Mediated Recombineering Enhances Metabolic Production of Kdo2-Lipid A by E. coli
In this article, we present an application of our novel E. coli CRISPR/Cas-mediated Lambda-Red (λ-Red) homologous recombination (HR) vector system, which facilitates gene editing through the homology-directed repair (HDR) of double-stranded DNA breaks (DSBs) created by Cas9 endonuclease, using either ssDNA or dsDNA as an editing template.
- Article: Ribonucleoprotein (RNP) Protocols Using Synthetic sgRNAs and Cas9 proteins
Combine guaranteed sgRNAs with our comprehensive range of CRISPR products and tools, including Cas9 and delivery reagents, for efficient genome modification with higher specificity.
- Article: Genome Editing in Plants with CRISPR/Cas9
Zinc finger nucleases (ZFNs), were introduced by Sigma-Aldrich less than 8 years ago, but in that time the technology of targeted genome editing has advanced rapidly. Most recently, the discovery of the CRISPR/Cas9 pathway has accelerated interest in this field, opening up new possibilities for research and development.
- Article: PURedit™ CRISPR Synthetic Guide RNAs and Cas9 Protein
Guaranteed PURedit™ CRISPR synthetic gRNAs and Cas9 protein offer industry-leading on-site cutting and specificity.
- eBook: Gene Editing 101
Learn how to understand, design and perform CRISPR experiments, as well as design gRNA, choose a Cas9 format, screen with CRISPR, use advanced CRISPR formats, and much more…
- Article: Blue-White Screening & Protocols for Colony Selection
Our popular blue-white screening for colony selection technical resource hub, transfection reagent selection guide and protocols.
- Article: Introduction to Cell Transfection
An overview on cell transfection vs transduction methods, our cell transfection reagent selection guide and protocols.
- Protocol: Western Blotting
Western blotting protocol that includes technical information supporting the following: Sample preparation, membrane transfer/tank transfer, transfer stack assembly, buffers, immunodetection, and a troubleshooting guide.
- Article: CRISPRi & CRISPRa Genetic Screening
了解我们用于基因敲低和过表达实验的优化 CRISPR活化和CRISPR抑制文库。我们的CRISPRi 和 SAM CRISPRa混合慢病毒文库可同时提供标准和定制选择。
- Article: Single-Cell Resolution Discovery
CRISPR lentiviral screening libraries, partnered with 10x Genomics, offer powerful research tools for pooled screening.
- Flyer: Determining Which Screening Option is Right for Your Experiment
Designing a screening experiment can be daunting with all the technologies and formats available. With the largest portfolio of screening tools on the market, we can help ensure you have the right tools at the right scale to meet your goals.
- Flyer: CRISPRi Whole Genome and Non-Coding Libraries
Lentiviral libraries and pools to take your research beyond the bench.
- Flyer: Whole Genome SAM CRISPRa Libraries
A complete kit for genome scale activation.
- Flyer: CRISPR Dual Guide Lentiviral Vectors
Double the editing power with dual gRNAs and integrated 10x genomics compatibility for single-cell analysis.
网络工具
- PCR Master Mix Calculator
Perform calculations for large scale PCR reactions and ensure success of scaled up reactions with this online PCR Master Mix Calculator.
- CRISPR Design Tool
Design your guide RNA for standard Cas9 editing and PURedit® Cas9 Cytosine Base Editor Proteins.
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