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Merck
CN

LVCAS9NEO

Sigma-Aldrich

Cas9 Neomycin Lenti Particles

Synonym(s):

Lenti Particles

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About This Item

UNSPSC Code:
41106609
NACRES:
NA.51
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packaging

vial of 200 μL

Quality Level

concentration

≥1 x 10 6 VP/ml virus (via p24 assay)

application(s)

CRISPR

selection

neomycin / G418

shipped in

dry ice

storage temp.

−70°C

General description

Ready-to-use Cas9 lentiviral particles are ideal for transducing a wide range of cell lines. The Cas9 Neomycin Lentiviral particles efficiently integrate Cas9 into the genomes of both dividing and non-dividing cell lines for use with CRISPR genome editing. Stably integrate Cas9 and Neo using the convenient, ready-to-use lentiviral particles while avoiding the tedious lentivirus production process.

Application

Functional Genomics/Screening /Target Validation/Genome Editing

Other Notes

Click here to order gRNA plasmid and virus.
200 ul of purified lentiviral particles (minimum of 1 X 106 TU/ml via a p24 assay) expressing Cas9 only with blasticidin resistance.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Articles

Genome-wide screening with optimized gRNAs per gene ensures specific and efficient knockout, controlling time and cost.

全基因组功能缺失筛查是发现生物过程背后的基因和途径的有效方法。现在,每个基因都可通过两个优化的 gRNA 完全敲除。通过最大限度减少克隆数量,可确保尽可能特异性的筛选,同时控制时间和成本。

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