Skip to Content
Merck
CN
Sign Into View Organizational & Contract Pricing

About This Item

EC Number:
3.4.21.50 (BRENDA, IUBMB)
UNSPSC Code:
12352204
NACRES:
NA.54
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Product Name

Lys-c (lysyl-endopeptidase), Active, from Achromobacter lyticus, recombinant, expressed in E. coli (206-473aa), tag-free, solution

biological source

Achromobacter lyticus

Quality Level

100
200

recombinant

expressed in E. coli (206-473aa)

grade

Proteomics Grade

form

ready-to-use solution

concentration

1 mg/mL

optimum pH

8.5

pH range

9.0-9.5(catalytic)

UniProt accession no.

P15636

shipped in

dry ice

storage temp.

−20°C

General description

Recombinant tag-free Achromobacter lyticus Lys-c (lysyl-endopeptidase) (206-473aa) was expressed in E.coli cells.

Overview
Lysyl-endopeptidase (Lys-c) was isolated from the Gram-negative soil bacterium Achromobacter lyticus by Msaki et al. The protein hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine and S-aminoethylcysteine residues making it an important tool for enzymatic protein sequencing and Lys-X compound synthesis.

Specificity

This enzyme hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine and S-aminoethylcysteine residues, at a catalytic pH range of 9.0-9.5, catalytic temperature range of 30-37 °C.

Application

The enzyme functions optimally between 30-37 °C and suffers from degradation when subjected to temperatures above 50 °C. Lysyl-endopeptidase retains complete activity after incubation in 4M urea or in 0.1% SDS solution for up to 6 hours at 30 °C.

Packaging

1mg/ml in Plastic

Preparation Note

Catalytic pH range 9.0-9.5. Catalytic temperature range 30-37 °C.

Storage and Stability

Store product at -20°C for up to one year. Avoid freeze/thaw cycles.

Other Notes

For R&D only.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
SLCQ6788
SLCQ6653
SLCQ4556
SLCJ4570

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service