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Merck
CN

M0535

Mycoplasma Broth

powder, suitable for microbiology

Synonym(s):

Mycoplasma media

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About This Item

NACRES:
NA.74
UNSPSC Code:
41106200

Key Documents

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Product Name

Mycoplasma Broth, powder, suitable for microbiology

form

powder

technique(s)

microbiological culture: suitable

solubility

H2O: 25.5 g/L (Autoclave at 121°C, 15 psi for 15 minutes. Store prepared medium at 0-5°C.)

application(s)

microbiology

Quality Level

200

Related Categories

Application

Mycoplasma Broth has been used for isolation and cultivation of mycoplasma. It may also be used to grow Porphyromonas gingivalis under anerobic conditions.

General description

Mycoplasma Broth is a basal medium which, after enrichment with yeast extract, horse serum, and antibiotics, will support the growth of Mycoplasma sp. The components contained in this basal medium do not exhibit inhibitory or toxic effects when used to culture mycoplasma. A special mineral supplement has been added to improve the growth and colony characteristics of the organism.

Other Notes

Powder contains (g/L): 10.0 bacterial peptone, 10.0 LAB-LEMCO powder, 5.0 sodium chloride, 0.5 mineral supplement.
The product is intended for laboratory use only.

Preparation Note

Basal Medium:

1. Suspend 25.5 g of powdered medium in 1L of distilled water. Stir until the powder dissolves.

2. Adjust the pH of the solution to 7.8 ± 0.2.

3. Autoclave at 121 °C and 15 psi for 15 minutes to sterilize. Cool to 50 °C and add sterile supplements.
Store dry powdered medium at room temperature and prepare medium at 2-8 °C.

Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

监管及禁止进口产品
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Certificates of Analysis (COA)

Lot/Batch Number
0000386626
0000351648
0000334603
0000318192
0000318192

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