Anti-Mouse IgG (Fab specific) F(ab′)₂ fragment antibody produced in goat

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases . Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice
Anti-Mouse IgG (Fab specific) F(ab′)₂ fragment antibody is monospecific as determined by immunoelectrophoresis versus normal mouse serum, mouse IgG and the Fab fragment of mouse IgG. The antibody does not react with the Fc fragment of mouse IgG. Moreover, no reactivity is observed with human κ or lambda light chain, IgG, IgA, IgM, IgD and IgE, bovine IgG and IgM, or horse IgG as determined by ELISA. In immunoelectrophoresis, the antibody preparation is found to consist only of the F(ab′)₂ fragment of goat IgG, where specific antisera is applied to goat IgG fragments. No contamination with goat IgG whole molecule is observed.

Mouse IgG

Anti-Mouse IgG (Fab specific) F(ab′)₂ fragment antibody is suitable for use in ELISA (using a conc. Of 2μg/mI (100 μg/well) in 50 mM carbonate/bicarbonate buffer, at pH9.6) . The antibody can also be used in immunoelectrophoresis.

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Antibody adsorbed with bovine, equine and human serum proteins.

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